anti-phosphothreonine rabbit mab

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Recombinant Rabbit Monoclonal Anti-Phosphothreonine Rabbit mAb Clone Number: PA-031-10 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: All Synonyms: pThr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms pThr UniProt ID / Immunogen MW (kDa) Multiple Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Protein phosphorylation is a common post-translational modification that can occur on many types of amino acids, such as tyrosine, serine, and threonine. The phosphorylation of threonine residues is related to many growth factors and oncogene protein kinases and plays an important role in the signal transduction of protein activation, cell proliferation and differentiation. The study of the transformation mechanism of oncogenes and growth factors in the mitogenic process depends on the identification of their substrates and subsequent determination of how phosphorylation affects their properties. The antibody that specifically recognizes amino acid phosphorylation can quickly identify, quantify, and immunoaffinityly separate cellular phosphorylated proteins, and can be used as an analysis and preparation tool, which is of inestimable value to the above-mentioned research. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 20 ng, 100 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate:(-): Jurkat, (+): Jurkat+ calyculin A (100nM, 30min)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: MultipleObserved band size: Multiple Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HeLa, (+): HeLa+ calyculin A (100nM, 15min)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: MultipleObserved band size: Multiple :
Recombinant Rabbit Monoclonal Anti-Phosphothreonine Rabbit mAb Clone Number: PA-031-10 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: All Synonyms: pThr Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms pThr UniProt ID / Immunogen MW (kDa) Multiple Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Protein phosphorylation is a common post-translational modification that can occur on many types of amino acids, such as tyrosine, serine, and threonine. The phosphorylation of threonine residues is related to many growth factors and oncogene protein kinases and plays an important role in the signal transduction of protein activation, cell proliferation and differentiation. The study of the transformation mechanism of oncogenes and growth factors in the mitogenic process depends on the identification of their substrates and subsequent determination of how phosphorylation affects their properties. The antibody that specifically recognizes amino acid phosphorylation can quickly identify, quantify, and immunoaffinityly separate cellular phosphorylated proteins, and can be used as an analysis and preparation tool, which is of inestimable value to the above-mentioned research. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Immunogen peptide quantity: 20 ng, 100 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate:(-): Jurkat, (+): Jurkat+ calyculin A (100nM, 30min)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: MultipleObserved band size: Multiple Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HeLa, (+): HeLa+ calyculin A (100nM, 15min)Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: MultipleObserved band size: Multiple