anti-phospho-p53 (ser15) mouse mab

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Anti-Phospho-p53 (Ser15) Mouse mAb Clone Number: 12041 Host: Mouse Clonality: Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: TP53 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms TP53 UniProt ID P04637 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Tumor protein P53, also known as p53, cellular tumor antigen p53, is an isoform of a protein encoded by homologous genes in various organisms. p53 is crucial in multicellular vertebrates, where it prevents cancer formation.As such, p53 has been described as “the guardian of the genome” because of its role in conserving stability by preventing genome mutation. Hence TP53 is classified as a tumor suppressor gene. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: HEK-293+Etoposide(-), 2: HEK-293 +Etoposide(+) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 44 kDa Observed band size: 53 kDa ICC/IF Cell line: HT-29 Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Description: The green color represents the positive signal observed with :
Anti-Phospho-p53 (Ser15) Mouse mAb Clone Number: 12041 Host: Mouse Clonality: Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: TP53 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms TP53 UniProt ID P04637 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Tumor protein P53, also known as p53, cellular tumor antigen p53, is an isoform of a protein encoded by homologous genes in various organisms. p53 is crucial in multicellular vertebrates, where it prevents cancer formation.As such, p53 has been described as “the guardian of the genome” because of its role in conserving stability by preventing genome mutation. Hence TP53 is classified as a tumor suppressor gene. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: HEK-293+Etoposide(-), 2: HEK-293 +Etoposide(+) Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 44 kDa Observed band size: 53 kDa ICC/IF Cell line: HT-29 Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Description: The green color represents the positive signal observed with