Product Name :
anti-phospho-histone h2b (tyr37) mouse mab
Isotype :
IgG1/Kappa
Conjugate :
Unconjugated
Synonyms:
H2BT37ph
UniProt ID :
Q8N257
Immunogen:
MW (kDa) :
Specificity:
Purity :
Protein G purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylation directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone phosphorylation occurs on serine, threonine and tyrosine residues on the amino-terminal of core histones. This histone mark plays roles in DNA repair, transcription and chromatin remodeling. The best-known histone phosphorylation site is histone variant H2A.x Ser139ph, which were reported to be involved in the response to DNA damage. Histone phosphorylation is mainly involved in processes during both mitosis and meiosis. Many kinases and phosphatases regulate histone phosphorylation levels. Cellular location Nucleus
Images :
WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb(HRP Conjugate)Lysate: 1: HepG2, 2: Neuro-2aProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 14 kDaObserved band size: 14 kDa IHC-P Tissue: Human liverSection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with
Vapor Pressure :
Anti-Phospho-Histone H2B (Tyr37) Mouse mAb Clone Number: 11001 Host: Mouse Clonality: Monoclonal Applications: WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: H2BT37ph Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/Kappa Conjugate Unconjugated Synonyms H2BT37ph UniProt ID Q8N257 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylation directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone phosphorylation occurs on serine, threonine and tyrosine residues on the amino-terminal of core histones. This histone mark plays roles in DNA repair, transcription and chromatin remodeling. The best-known histone phosphorylation site is histone variant H2A.x Ser139ph, which were reported to be involved in the response to DNA damage. Histone phosphorylation is mainly involved in processes during both mitosis and meiosis. Many kinases and phosphatases regulate histone phosphorylation levels. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb(HRP Conjugate)Lysate: 1: HepG2, 2: Neuro-2aProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 14 kDaObserved band size: 14 kDa IHC-P Tissue: Human liverSection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with
Anti-Phospho-Histone H2B (Tyr37) Mouse mAb Clone Number: 11001 Host: Mouse Clonality: Monoclonal Applications: WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: H2BT37ph Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/Kappa Conjugate Unconjugated Synonyms H2BT37ph UniProt ID Q8N257 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylation directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone phosphorylation occurs on serine, threonine and tyrosine residues on the amino-terminal of core histones. This histone mark plays roles in DNA repair, transcription and chromatin remodeling. The best-known histone phosphorylation site is histone variant H2A.x Ser139ph, which were reported to be involved in the response to DNA damage. Histone phosphorylation is mainly involved in processes during both mitosis and meiosis. Many kinases and phosphatases regulate histone phosphorylation levels. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb(HRP Conjugate)Lysate: 1: HepG2, 2: Neuro-2aProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 14 kDaObserved band size: 14 kDa IHC-P Tissue: Human liverSection type: Formalin fixed & Paraffin – embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with
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