anti-phospho-ampk beta 1 (ser182) rabbit pab

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Anti-Phospho-AMPK beta 1 (Ser182) Rabbit pAb Clone Number: – Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human Synonyms: AMPK subunit beta-1, AMPKb, AMPK, PRKAB1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms AMPK subunit beta-1, AMPKb, AMPK, PRKAB1 UniProt ID Q9Y478 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background AMPKβ1 is the β1 regulating subunit of AMP-activated protein kinase. It is post-translationally modified by myristoylation and multi-site phosphorylation including Ser24/25, Ser96, Ser101, Ser108, and Ser182. Phosphorylation at Ser108 of the β1 subunit seems to be required for AMPK activation, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization. Several mutations in AMPKγ subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumulation in heart or skeletal muscle. Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS. Cellular location Cell nucleus and cytosol Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000 Primary Ab incubation condition: 2hoursat room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: (-): HeLa, (+): HeLa+ OkadaicA(100nM,60min) + Calyculin A (100nM,60min) Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 38 kDa Observed band size: 38 kDa :
Anti-Phospho-AMPK beta 1 (Ser182) Rabbit pAb Clone Number: – Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human Synonyms: AMPK subunit beta-1, AMPKb, AMPK, PRKAB1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms AMPK subunit beta-1, AMPKb, AMPK, PRKAB1 UniProt ID Q9Y478 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background AMPKβ1 is the β1 regulating subunit of AMP-activated protein kinase. It is post-translationally modified by myristoylation and multi-site phosphorylation including Ser24/25, Ser96, Ser101, Ser108, and Ser182. Phosphorylation at Ser108 of the β1 subunit seems to be required for AMPK activation, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization. Several mutations in AMPKγ subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumulation in heart or skeletal muscle. Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS. Cellular location Cell nucleus and cytosol Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000 Primary Ab incubation condition: 2hoursat room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: (-): HeLa, (+): HeLa+ OkadaicA(100nM,60min) + Calyculin A (100nM,60min) Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 38 kDa Observed band size: 38 kDa