anti-alix mouse mab

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Recombinant Anti-ALIX Mouse mAb Clone Number: 11720 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: AIP1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms AIP1 UniProt ID Q8WUM4 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Programmed cell death 6-interacting protein also known as ALIX is a protein that in humans is encoded by the PDCD6IP gene. This gene encodes a protein thought to participate in programmed cell death. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death. Cellular location Cytoplasm, Secreted Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: SH-SY5YProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 100 kDaObserved band size: 100 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: JurKatProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 100 kDaObserved band size: 100 kDa :
Recombinant Anti-ALIX Mouse mAb Clone Number: 11720 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: AIP1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms AIP1 UniProt ID Q8WUM4 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Programmed cell death 6-interacting protein also known as ALIX is a protein that in humans is encoded by the PDCD6IP gene. This gene encodes a protein thought to participate in programmed cell death. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death. Cellular location Cytoplasm, Secreted Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: SH-SY5YProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 100 kDaObserved band size: 100 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: JurKatProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 100 kDaObserved band size: 100 kDa