anti-periostin mouse mab

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Recombinant Anti-Periostin Mouse mAb Clone Number: JMMR-2087 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse Synonyms: Osteoblast-specific factor 2, PN, OSF-2, POSTN Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Osteoblast-specific factor 2, PN, OSF-2, POSTN UniProt ID Q15063 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse IHC-P 1:50 – 1:200 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Periostin (POSTN, PN), originally named as osteoblast-specific factor 2 (OSF-2), is a secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues, and has a role in bone, tooth and heart development and function. Periostin is overexpressed in a broad range of human cancer types, including lung, ovary, breast and colon cancers. Periostin is also expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour, and it is required to allow cancer stem cell maintenance. Cellular location Secreted, Golgi apparatus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4℃ overnight Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: HeLa, 2: 4T1, 3: BxPC-3 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 80 kDa Observed band size: 75 kDa IHC-P Tissue: Human colon Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:200 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Anti-Periostin Mouse mAb Clone Number: JMMR-2087 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse Synonyms: Osteoblast-specific factor 2, PN, OSF-2, POSTN Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Osteoblast-specific factor 2, PN, OSF-2, POSTN UniProt ID Q15063 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse IHC-P 1:50 – 1:200 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Periostin (POSTN, PN), originally named as osteoblast-specific factor 2 (OSF-2), is a secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues, and has a role in bone, tooth and heart development and function. Periostin is overexpressed in a broad range of human cancer types, including lung, ovary, breast and colon cancers. Periostin is also expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour, and it is required to allow cancer stem cell maintenance. Cellular location Secreted, Golgi apparatus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4℃ overnight Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: HeLa, 2: 4T1, 3: BxPC-3 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 80 kDa Observed band size: 75 kDa IHC-P Tissue: Human colon Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:200 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with