anti-pdcd4 mouse mab

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Recombinant Anti-PDCD4 Mouse mAb Clone Number: JMMR-1367 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human Synonyms: Protein 197/15a, H731 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Protein 197/15a, H731 UniProt ID Q53EL6 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background PDCD4 was a suppressor of neoplastic transformation. It interacts with eukaryotic initiation factors eIF4AI and eIF4AII. PDCD4 colocalizes with eIF4A in the cytoplasm and inhibits the activity of eIF4A as an ATP-dependent RNA helicase. PDCD4 inhibits cap-dependent translation. Upon stimulation by mitogens, PDCD4 is phosphorylated on Ser67 by S6K1 kinase and then degraded through the ubiquitin pathway leading to enhanced translation and cell growth. Cellular location Cytosol, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: HeLa, 2: Jurkat, 3: HEK-293TProtein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 52 kDa Observed band size: 56 kDa IHC-P Tissue: Human breast carcinoma Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Anti-PDCD4 Mouse mAb Clone Number: JMMR-1367 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human Synonyms: Protein 197/15a, H731 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Protein 197/15a, H731 UniProt ID Q53EL6 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background PDCD4 was a suppressor of neoplastic transformation. It interacts with eukaryotic initiation factors eIF4AI and eIF4AII. PDCD4 colocalizes with eIF4A in the cytoplasm and inhibits the activity of eIF4A as an ATP-dependent RNA helicase. PDCD4 inhibits cap-dependent translation. Upon stimulation by mitogens, PDCD4 is phosphorylated on Ser67 by S6K1 kinase and then degraded through the ubiquitin pathway leading to enhanced translation and cell growth. Cellular location Cytosol, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: HeLa, 2: Jurkat, 3: HEK-293TProtein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 52 kDa Observed band size: 56 kDa IHC-P Tissue: Human breast carcinoma Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with