anti-pd-l1 rabbit mab

Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-PD-L1 Rabbit mAb Clone Number: 10479 Host: Rabbit Clonality: Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: CD274, B7H1, PDCD1L1, PDCD1LG1 Product Size 100μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms CD274, B7H1, PDCD1L1, PDCD1LG1 UniProt ID Q9NZQ7 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:1000 Human IHC-P 1:50-1:100 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Programmed death-ligand 1 (PD-L1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a protein that in humans is encoded by the CD274 gene. Programmed death-ligand 1 (PD-L1) is a type 1 transmembrane protein that has been speculated to play a major role in suppressing the adaptive arm of immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. The binding of PD-L1 to the inhibitory checkpoint molecule PD-1 transmits an inhibitory signal based on interaction with phosphatases (SHP-1 or SHP-2) via Immunoreceptor Tyrosine-Based Switch Motif (ITSM).This reduces the proliferation of antigen-specific T-cells in lymph nodes, while simultaneously reducing apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells) – further mediated by a lower regulation of the gene Bcl-2. Cellular location Cell membrane, Endosome Images WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: Human placenta, HT-29+IFN_x0002_gamma(-)(1mg/ml), HT-29+IFN_x0002_gamma(+)(1mg/ml), PC-3Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 50 kDaObserved MW: 50 kDa IHC-P Tissue: Human lung squamous carcinomaSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100Primary ab incubation condition: 1 hour at room temperature Secondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)Counter stain: Hematoxylin (Blue)Comment: Color brown is the positive signal for :
Recombinant Rabbit Monoclonal Anti-PD-L1 Rabbit mAb Clone Number: 10479 Host: Rabbit Clonality: Monoclonal Applications： WB IHC-P Reactivity: Human Synonyms: CD274, B7H1, PDCD1L1, PDCD1LG1 Product Size 100μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms CD274, B7H1, PDCD1L1, PDCD1LG1 UniProt ID Q9NZQ7 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:1000 Human IHC-P 1:50-1:100 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Programmed death-ligand 1 (PD-L1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a protein that in humans is encoded by the CD274 gene. Programmed death-ligand 1 (PD-L1) is a type 1 transmembrane protein that has been speculated to play a major role in suppressing the adaptive arm of immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. The binding of PD-L1 to the inhibitory checkpoint molecule PD-1 transmits an inhibitory signal based on interaction with phosphatases (SHP-1 or SHP-2) via Immunoreceptor Tyrosine-Based Switch Motif (ITSM).This reduces the proliferation of antigen-specific T-cells in lymph nodes, while simultaneously reducing apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells) – further mediated by a lower regulation of the gene Bcl-2. Cellular location Cell membrane, Endosome Images WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: Human placenta, HT-29+IFN_x0002_gamma(-)(1mg/ml), HT-29+IFN_x0002_gamma(+)(1mg/ml), PC-3Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: 50 kDaObserved MW: 50 kDa IHC-P Tissue: Human lung squamous carcinomaSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100Primary ab incubation condition: 1 hour at room temperature Secondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use)Counter stain: Hematoxylin (Blue)Comment: Color brown is the positive signal for