anti-aldolase a rabbit pab

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Anti-Aldolase A Rabbit pAb Clone Number: JRMR-408 Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDOA Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDOA UniProt ID P04075 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human, Mouse, Rat ICC/IF 1:50 Human, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Aldolase A is also named as lung cancer antigen NY-LU-1, muscle-type aldolase, Aldolase A and belongs to the class I fructose-bisphosphate aldolase family. It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. Aldolase A is upregulated in various cancers, including human lung squamous and renal cell carcinomas. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: HEK-293, 3: MCF-7, 4: HepG2, 5: C2C12, 6: NIH/3T3Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 39 kDaObserved band size: 39 kDa IHC-P Tissue: Human colon carcinornaSection type: Formalin-fixed & Paraffinembedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Anti-Aldolase A Rabbit pAb Clone Number: JRMR-408 Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDOA Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDOA UniProt ID P04075 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human, Mouse, Rat ICC/IF 1:50 Human, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Aldolase A is also named as lung cancer antigen NY-LU-1, muscle-type aldolase, Aldolase A and belongs to the class I fructose-bisphosphate aldolase family. It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. Aldolase A is upregulated in various cancers, including human lung squamous and renal cell carcinomas. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: HEK-293, 3: MCF-7, 4: HepG2, 5: C2C12, 6: NIH/3T3Protein loading quantity: 20 μgExposure time: 30 sPredicted band size: 39 kDaObserved band size: 39 kDa IHC-P Tissue: Human colon carcinornaSection type: Formalin-fixed & Paraffinembedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with