anti-pacsin3 rabbit mab

Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-PACSIN3 Rabbit mAb Clone Number: JRMR-11075-2 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF FC Reactivity: Human Synonyms: SH3 domain-containing protein 6511 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms SH3 domain-containing protein 6511 UniProt ID Q9UKS6 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human ICC/IF 1:50 Human FC 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Protein kinase C and casein kinase substrate in neurons 3 (PACSIN3) Plays a role in endocytosis and regulates internalization of plasma membrane proteins. PACSIN3 overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. PACSIN3 inhibits the TRPV4 calcium channel activity. Cellular location Cell membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate:1: HepG2, 2: HeLa, 3: Human heartProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 48 kDaObserved band size: 48 kDa ICC/IF Cell line: MCF7Fixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-PACSIN3 Rabbit mAb Clone Number: JRMR-11075-2 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF FC Reactivity: Human Synonyms: SH3 domain-containing protein 6511 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms SH3 domain-containing protein 6511 UniProt ID Q9UKS6 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 Human ICC/IF 1:50 Human FC 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Protein kinase C and casein kinase substrate in neurons 3 (PACSIN3) Plays a role in endocytosis and regulates internalization of plasma membrane proteins. PACSIN3 overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. PACSIN3 inhibits the TRPV4 calcium channel activity. Cellular location Cell membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate:1: HepG2, 2: HeLa, 3: Human heartProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 48 kDaObserved band size: 48 kDa ICC/IF Cell line: MCF7Fixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with