anti-p21 rabbit mab

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Recombinant Rabbit Monoclonal Anti-p21 Rabbit mAb Clone Number: JRMR-10499(2)-22 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P IP Reactivity: Human Synonyms: MDA-6, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms MDA-6, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1 UniProt ID P38936 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:200 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background This protein is a potent cyclin-dependent kinase inhibitor, which binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus as a regulator of cell cycle progression at G1. The expression of this protein is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: MCF7, 2: U87-MGProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 18 kDaObserved band size: 18 kDa IHC-P Tissue: Human cervical carcinomaSection type: Formalin-fixed & Paraffin_x005f_x0002_embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-p21 Rabbit mAb Clone Number: JRMR-10499(2)-22 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P IP Reactivity: Human Synonyms: MDA-6, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms MDA-6, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1 UniProt ID P38936 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human IHC-P 1:200 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background This protein is a potent cyclin-dependent kinase inhibitor, which binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus as a regulator of cell cycle progression at G1. The expression of this protein is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: MCF7, 2: U87-MGProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 18 kDaObserved band size: 18 kDa IHC-P Tissue: Human cervical carcinomaSection type: Formalin-fixed & Paraffin_x005f_x0002_embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with