anti-myosin heavy chain 1 mouse mab

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Recombinant Anti-Myosin Heavy Chain 1 Mouse mAb Clone Number: 10535 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Myosin-1, MyHC-2x, MyHC-IIx/d, MYH1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms Myosin-1, MyHC-2x, MyHC-IIx/d, MYH1 UniProt ID P12882 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:100000-1:500000 Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Myosin heavy chain 1, also known as striated muscle myosin heavy chain 1, is a protein that is encoded by the MYH1 gene in human. This gene is most highly expressed in fast type IIX/D muscle fibres of vertebrates and encodes a protein found uniquely in striated muscle. Myosin is a major contractile protein that converts chemical energy into mechanical energy through the hydrolysis of ATP. Cellular location Cytoplasm, Myofibrils Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:50wPrimary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: Mouse skeletal muscle, 2: Rat skeletal muscleProtein loading quantity: 1 μgExposure time: 30 sPredicted band size: 223 kDaObserved band size: 250 kDa IHC-P Tissue: Human skeletal muscleSection type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Anti-Myosin Heavy Chain 1 Mouse mAb Clone Number: 10535 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Myosin-1, MyHC-2x, MyHC-IIx/d, MYH1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms Myosin-1, MyHC-2x, MyHC-IIx/d, MYH1 UniProt ID P12882 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:100000-1:500000 Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Myosin heavy chain 1, also known as striated muscle myosin heavy chain 1, is a protein that is encoded by the MYH1 gene in human. This gene is most highly expressed in fast type IIX/D muscle fibres of vertebrates and encodes a protein found uniquely in striated muscle. Myosin is a major contractile protein that converts chemical energy into mechanical energy through the hydrolysis of ATP. Cellular location Cytoplasm, Myofibrils Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:50wPrimary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1: Mouse skeletal muscle, 2: Rat skeletal muscleProtein loading quantity: 1 μgExposure time: 30 sPredicted band size: 223 kDaObserved band size: 250 kDa IHC-P Tissue: Human skeletal muscleSection type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with