anti-myo1c rabbit mab

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Recombinant Rabbit Monoclonal Anti-MYO1C Rabbit mAb Clone Number: JMMR-3654 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: Unconventional myosin-Ic, MMI-beta, MMIb Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Unconventional myosin-Ic, MMI-beta, MMIb UniProt ID O00159 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Myosin 1C (MYO1C) is a member of the unconventional myosin protein family, which are actin-based molecular motors. The protein is found in the cytoplasm, and one isoform with a unique N-terminus is also found in the nucleus. The nuclear isoform associates with RNA polymerase I and II and functions in transcription initiation. The mouse ortholog of this protein also functions in intracellular vesicle transport to the plasma membrane. The related gene myosin 1E has been referred to as myosin 1C in the literature, but it is a distinct locus on chromosome 19. Cellular location Cytoplasm, Nucleus, membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: HeLa; 2: HepG2; 3: A431; 4: Mouseheart; 5: C2C12; 6: C6 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 122 kDa Observed band size: 122 kDa IHC-P Tissue: Human tonsil Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-MYO1C Rabbit mAb Clone Number: JMMR-3654 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF Reactivity: Human, Mouse, Rat Synonyms: Unconventional myosin-Ic, MMI-beta, MMIb Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Unconventional myosin-Ic, MMI-beta, MMIb UniProt ID O00159 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Myosin 1C (MYO1C) is a member of the unconventional myosin protein family, which are actin-based molecular motors. The protein is found in the cytoplasm, and one isoform with a unique N-terminus is also found in the nucleus. The nuclear isoform associates with RNA polymerase I and II and functions in transcription initiation. The mouse ortholog of this protein also functions in intracellular vesicle transport to the plasma membrane. The related gene myosin 1E has been referred to as myosin 1C in the literature, but it is a distinct locus on chromosome 19. Cellular location Cytoplasm, Nucleus, membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: HeLa; 2: HepG2; 3: A431; 4: Mouseheart; 5: C2C12; 6: C6 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 122 kDa Observed band size: 122 kDa IHC-P Tissue: Human tonsil Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with