anti-myh7 rabbit pab

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Anti-MYH7 Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: Myosin-7, MyHC-slow, MyHC-beta, MYH7, MYHCB, Slow skeletal myosin heavy chain Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Myosin-7, MyHC-slow, MyHC-beta, MYH7, MYHCB, Slow skeletal myosin heavy chain UniProt ID P12883 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000-3000 Human, Rat, Mouse Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively. While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions. The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization. Research studies have shown that phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:3000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: Human heart Protein loading amount: 5 μg Exposure time: 10 secondsPredicted band size: 223 kDa Observed band size: 223 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: Rat heart Protein loading amount: 20 μg Exposure time: 3 s Predicted band size: 223 kDa Observed band size: 223 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: Mouse skeletal muscle, 2: Rat skeletal muscle Protein loading amount: 20 μg Exposure time: 60 secondsPredicted band size: 223 kDa Observed band size: 223 kDa :
Anti-MYH7 Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB Reactivity: Human, Mouse, Rat Synonyms: Myosin-7, MyHC-slow, MyHC-beta, MYH7, MYHCB, Slow skeletal myosin heavy chain Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Myosin-7, MyHC-slow, MyHC-beta, MYH7, MYHCB, Slow skeletal myosin heavy chain UniProt ID P12883 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000-3000 Human, Rat, Mouse Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively. While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions. The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization. Research studies have shown that phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration. Cellular location Cytoplasm Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:3000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: Human heart Protein loading amount: 5 μg Exposure time: 10 secondsPredicted band size: 223 kDa Observed band size: 223 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: Rat heart Protein loading amount: 20 μg Exposure time: 3 s Predicted band size: 223 kDa Observed band size: 223 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: Mouse skeletal muscle, 2: Rat skeletal muscle Protein loading amount: 20 μg Exposure time: 60 secondsPredicted band size: 223 kDa Observed band size: 223 kDa