anti-monomethyl-histone h4 (lys20) mouse mab

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Recombinant Anti-Monomethyl-Histone H4 (Lys20) Mouse mAb Clone Number: 12425 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF ChIP Reactivity: Human, Mouse, Rat Synonyms: H4K20me1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/Kappa Conjugate Unconjugated Synonyms H4K20me1 UniProt ID P62805 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ICC/IF 1:50 – 1:100 Human ChIP 6 μg/5×106 cells Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, Trimethylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: HeLa, N2a, BRL, Mouse spleen, Mouse kidneyProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 11 kDaObserved band size: 11 kDa ICC/IF Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 0.1% Triton X-100Primary Ab dilution: 1:100Primary incubation condition: 1 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Counter stain: Tubulin (Red)Description: The green color represents the positive signal observed with :
Recombinant Anti-Monomethyl-Histone H4 (Lys20) Mouse mAb Clone Number: 12425 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF ChIP Reactivity: Human, Mouse, Rat Synonyms: H4K20me1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/Kappa Conjugate Unconjugated Synonyms H4K20me1 UniProt ID P62805 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ICC/IF 1:50 – 1:100 Human ChIP 6 μg/5×106 cells Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, Trimethylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: HeLa, N2a, BRL, Mouse spleen, Mouse kidneyProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 11 kDaObserved band size: 11 kDa ICC/IF Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 0.1% Triton X-100Primary Ab dilution: 1:100Primary incubation condition: 1 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Counter stain: Tubulin (Red)Description: The green color represents the positive signal observed with