anti-monomethyl-histone h3 (lys27) rabbit mab

Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-Monomethyl-Histone H3 (Lys27) Rabbit mAb Clone Number: PA-174-18 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF ChIP Reactivity: Human, Mouse, Rat, Pig, Corn Synonyms: H3K27me1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K27me1 UniProt ID P68431 Immunogen Monomethylated human histone H3 (Lys27) peptide MW (kDa) 15 Specificity This antibody detects histone H3 only when it is Monomethylated at Lys27, but not the H3 peptide Monomethylated at Lys9 and Lys23, dimethylated at Lys27 and Lys9, Trimethylated at Lys27 and Lys 23, and the unmodified H3 peptide at Lys27. Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:2000 Human, Mouse, Rat, Pig, Corn IHC-P 1:1000 – 1:3000 Human, Mouse ICC/IF 1:500- 1:1000 Human ChIP 6 μg/5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation,phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, trimethylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20,while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-rabbit IgG H&L (HRP) Peptide amount: 1 ng, 4 ng, 16 ng, 64ng Exposure time: 30 s The list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 4℃ overnightSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: Neuro-2a, 3: BRL, 4: Mouse liver, 5: Rat brain, 6: Pig brain, 7: Recombinant histone H3 (50ng)Protein loading amount: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4℃ overnightSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: Maize leavesProtein loading amount: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa IHC-P Tissue: Human testis Section type: Formalin-fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:3000Primary Ab incubation condition: 1 hour at room temperatureSecondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (blue)Description: Color brown is the positive signal for :
Recombinant Rabbit Monoclonal Anti-Monomethyl-Histone H3 (Lys27) Rabbit mAb Clone Number: PA-174-18 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF ChIP Reactivity: Human, Mouse, Rat, Pig, Corn Synonyms: H3K27me1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K27me1 UniProt ID P68431 Immunogen Monomethylated human histone H3 (Lys27) peptide MW (kDa) 15 Specificity This antibody detects histone H3 only when it is Monomethylated at Lys27, but not the H3 peptide Monomethylated at Lys9 and Lys23, dimethylated at Lys27 and Lys9, Trimethylated at Lys27 and Lys 23, and the unmodified H3 peptide at Lys27. Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:2000 Human, Mouse, Rat, Pig, Corn IHC-P 1:1000 – 1:3000 Human, Mouse ICC/IF 1:500- 1:1000 Human ChIP 6 μg/5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation,phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, trimethylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20,while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-rabbit IgG H&L (HRP) Peptide amount: 1 ng, 4 ng, 16 ng, 64ng Exposure time: 30 s The list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 4℃ overnightSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: Neuro-2a, 3: BRL, 4: Mouse liver, 5: Rat brain, 6: Pig brain, 7: Recombinant histone H3 (50ng)Protein loading amount: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4℃ overnightSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: Maize leavesProtein loading amount: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa IHC-P Tissue: Human testis Section type: Formalin-fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:3000Primary Ab incubation condition: 1 hour at room temperatureSecondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (blue)Description: Color brown is the positive signal for