anti-methacryllysine mouse mab

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Recombinant Anti-Methacryllysine Mouse mAb Clone Number: 3-1-4 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: All Synonyms: Kmea Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kmea UniProt ID / Immunogen Methacrylated lysine peptides MW (kDa) Multiple Specificity Anti-Methacryllysine Mouse mAb detects proteins post-translationally modified by methacrylation on lysine residues. This pan antibody recognizes methacrylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All IHC-P 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Histone lysine methylacrylylation (Kmea) is a novel post-translational modification. It is a structural isomer of crotonyllysine, with a different mechanism and function. Specifically, methacrylate is the metabolic precursor of Kmea, and HAT1, SIRT1 and SIRT2 are the writer and erasers of Kmea, respectively. In addition, 27 histone Kmea sites were identified in HeLa cells. Leigh syndrome (LS) is a neurological disease characterized by mitochondrial defects. Accumulation of methacrylyl-coA is identified in LS patients with genetic mutations in short-chain enoyl-CoA hydratase (ECHS1) and 3-hydroxyisobutyryl-CoA hydrolase (HIBCH) in the metabolic pathway of valine.The discovery of Kmea suggests a new direction for the pathological role of methylacrylyl-coA accumulation. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Peptide amount: 1: 4ng, 20 ng, 100 ng; 2-6: 100 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1-4: HeLa, MCF-7, 293T, N2aProtein loading amount: 20 μgExposure time: 60 sPredicted band size: MultipleObserved band size: Multiple IHC-P Tissue: Human cerebrum Section type: Formalin-fixed & paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:2000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with :
Recombinant Anti-Methacryllysine Mouse mAb Clone Number: 3-1-4 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: All Synonyms: Kmea Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kmea UniProt ID / Immunogen Methacrylated lysine peptides MW (kDa) Multiple Specificity Anti-Methacryllysine Mouse mAb detects proteins post-translationally modified by methacrylation on lysine residues. This pan antibody recognizes methacrylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All IHC-P 1:500 – 1:2000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Histone lysine methylacrylylation (Kmea) is a novel post-translational modification. It is a structural isomer of crotonyllysine, with a different mechanism and function. Specifically, methacrylate is the metabolic precursor of Kmea, and HAT1, SIRT1 and SIRT2 are the writer and erasers of Kmea, respectively. In addition, 27 histone Kmea sites were identified in HeLa cells. Leigh syndrome (LS) is a neurological disease characterized by mitochondrial defects. Accumulation of methacrylyl-coA is identified in LS patients with genetic mutations in short-chain enoyl-CoA hydratase (ECHS1) and 3-hydroxyisobutyryl-CoA hydrolase (HIBCH) in the metabolic pathway of valine.The discovery of Kmea suggests a new direction for the pathological role of methylacrylyl-coA accumulation. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Peptide amount: 1: 4ng, 20 ng, 100 ng; 2-6: 100 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: 1-4: HeLa, MCF-7, 293T, N2aProtein loading amount: 20 μgExposure time: 60 sPredicted band size: MultipleObserved band size: Multiple IHC-P Tissue: Human cerebrum Section type: Formalin-fixed & paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:2000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with