anti-methacryl-histone h3 (lys18) mouse mab

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Recombinant Anti-Methacryl-Histone H3 (Lys18) Mouse mAb Clone Number: 202-7(G76) Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: H3K18mea Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K18mea UniProt ID P68431 Immunogen Methacrylated human histone H3 (Lys18) peptide MW (kDa) 15 Specificity Anti-Methacryl-Histone H3 (Lys18) Mouse mAb detects histone H3 only when it is methacrylated at Lys18. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Histone lysine methylacrylylation (Kmea) is a novel post-translational modification. It is a structural isomer of crotonyllysine, with a different mechanism and function. Specifically, methacrylate is the metabolic precursor of Kmea, and HAT1, SIRT1 and SIRT2 are the writer and erasers of Kmea, respectively. In addition, 27 histone Kmea sites were identified in HeLa cells. Leigh syndrome (LS) is a neurological disease characterized by mitochondrial defects. Accumulation of methacrylyl-coA is identified in LS patients with genetic mutations in short-chain enoyl-CoA hydratase (ECHS1) and 3-hydroxyisobutyryl-CoA hydrolase (HIBCH) in the metabolic pathway of valine.The discovery of Kmea suggests a new direction for the pathological role of methylacrylyl-coA accumulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Peptide amount: 1-2: 1ng, 4 ng, 16 ng, 64 ng 3-16: 64ng Exposure time: 30 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: (-): HeLa; (+): HeLa + sodium butyrate (30 mM, 4 hours) Protein loading amount: 20 μgExposure time: 30 secondsPredicted band size: 15 kDa Observed band size: 15 kDa :
Recombinant Anti-Methacryl-Histone H3 (Lys18) Mouse mAb Clone Number: 202-7(G76) Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: H3K18mea Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K18mea UniProt ID P68431 Immunogen Methacrylated human histone H3 (Lys18) peptide MW (kDa) 15 Specificity Anti-Methacryl-Histone H3 (Lys18) Mouse mAb detects histone H3 only when it is methacrylated at Lys18. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. Histone lysine methylacrylylation (Kmea) is a novel post-translational modification. It is a structural isomer of crotonyllysine, with a different mechanism and function. Specifically, methacrylate is the metabolic precursor of Kmea, and HAT1, SIRT1 and SIRT2 are the writer and erasers of Kmea, respectively. In addition, 27 histone Kmea sites were identified in HeLa cells. Leigh syndrome (LS) is a neurological disease characterized by mitochondrial defects. Accumulation of methacrylyl-coA is identified in LS patients with genetic mutations in short-chain enoyl-CoA hydratase (ECHS1) and 3-hydroxyisobutyryl-CoA hydrolase (HIBCH) in the metabolic pathway of valine.The discovery of Kmea suggests a new direction for the pathological role of methylacrylyl-coA accumulation. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Peptide amount: 1-2: 1ng, 4 ng, 16 ng, 64 ng 3-16: 64ng Exposure time: 30 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: (-): HeLa; (+): HeLa + sodium butyrate (30 mM, 4 hours) Protein loading amount: 20 μgExposure time: 30 secondsPredicted band size: 15 kDa Observed band size: 15 kDa