anti-mcm2 rabbit mab

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Recombinant Rabbit Monoclonal Anti-MCM2 Rabbit mAb Clone Number: JRMR-10277-36 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, BM28, CCNL1, CDCL1, KIAA0030 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, BM28, CCNL1, CDCL1, KIAA0030 UniProt ID P49736 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background MCM2 is a nuclear protein that plays an important role in the key steps of DNA replication and cell division in the cell cycle. The content of MCM2 is very low in cells in the quiescent phase, while in proliferating and transformed cells, the content of MCM2 begins to increase in the G0 phase, reaches a peak at the end of G0 and early s, and binds to chromatin, and is free from the s phase to the M phase. MCM2, G2 phase and M phase decreased. Due to this cyclical change consistent with the cell proliferation process, MCM2 has now been regarded as a marker for cells in the s phase, and it is also a specific proliferation-related factor, reflecting the cell proliferation activity. MCM2 protein can distinguish the corresponding normal tissues, dysplastic tissues and tumor tissues. In reproductive system tumors, gastric cancer, and other cancer tissues, the expression index of MCM2 protein is positively correlated with histological grade and Nottingham prognostic index, that is: the higher the MCM2 protein expression index, the higher the malignancy of the tumor, and the patient’s The worse the prognosis, the greater the risk of death, and it has more predictive significance than Ki67. In short, MCM2 protein is used in clinic as a cell proliferation marker, and it has certain significance for the diagnosis and prognosis of precancerous lesions and malignant tumors. Cellular location Chromosome, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: SW480, 3: MCF-7, 4: PC_x0002_12, 5: NIH-3T3Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 102 kDaObserved band size: 125 kDa IHC-P Tissue: Human tonsilSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-MCM2 Rabbit mAb Clone Number: JRMR-10277-36 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, BM28, CCNL1, CDCL1, KIAA0030 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, BM28, CCNL1, CDCL1, KIAA0030 UniProt ID P49736 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:200 – 1:1000 Human, Mouse, Rat Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background MCM2 is a nuclear protein that plays an important role in the key steps of DNA replication and cell division in the cell cycle. The content of MCM2 is very low in cells in the quiescent phase, while in proliferating and transformed cells, the content of MCM2 begins to increase in the G0 phase, reaches a peak at the end of G0 and early s, and binds to chromatin, and is free from the s phase to the M phase. MCM2, G2 phase and M phase decreased. Due to this cyclical change consistent with the cell proliferation process, MCM2 has now been regarded as a marker for cells in the s phase, and it is also a specific proliferation-related factor, reflecting the cell proliferation activity. MCM2 protein can distinguish the corresponding normal tissues, dysplastic tissues and tumor tissues. In reproductive system tumors, gastric cancer, and other cancer tissues, the expression index of MCM2 protein is positively correlated with histological grade and Nottingham prognostic index, that is: the higher the MCM2 protein expression index, the higher the malignancy of the tumor, and the patient’s The worse the prognosis, the greater the risk of death, and it has more predictive significance than Ki67. In short, MCM2 protein is used in clinic as a cell proliferation marker, and it has certain significance for the diagnosis and prognosis of precancerous lesions and malignant tumors. Cellular location Chromosome, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: SW480, 3: MCF-7, 4: PC_x0002_12, 5: NIH-3T3Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 102 kDaObserved band size: 125 kDa IHC-P Tissue: Human tonsilSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with