anti-mcm2 rabbit mab

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Recombinant Rabbit Monoclonal Anti-MCM2 Rabbit mAb Clone Number: JRMR-127 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF IP Reactivity: Human, Mouse, Rat Synonyms: Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, BM28, CCNL1, CDCL1, KIAA0030 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, BM28, CCNL1, CDCL1, KIAA0030 UniProt ID P49736 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human IHC-P 1:200 – 1:1000 Human, Mouse, Rat ICC/IF 1:100 – 1:500 Human IP 1:25 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for ‘once per cell cycle’ DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureLysate: HeLaProtein loading quantity: 20 μgExposure time: 15 sPredicted band size: 102 kDaObserved band size: 125 kDa IHC-P Tissue: Human cervical carcinomaSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Counter stain: HematoxylinDescription: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-MCM2 Rabbit mAb Clone Number: JRMR-127 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF IP Reactivity: Human, Mouse, Rat Synonyms: Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, BM28, CCNL1, CDCL1, KIAA0030 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, BM28, CCNL1, CDCL1, KIAA0030 UniProt ID P49736 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human IHC-P 1:200 – 1:1000 Human, Mouse, Rat ICC/IF 1:100 – 1:500 Human IP 1:25 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for ‘once per cell cycle’ DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureLysate: HeLaProtein loading quantity: 20 μgExposure time: 15 sPredicted band size: 102 kDaObserved band size: 125 kDa IHC-P Tissue: Human cervical carcinomaSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000Primary Ab incubation condition: 1 hour at room temperature Counter stain: HematoxylinDescription: The brown color represents the positive signal observed with