anti-malonyl-histone h3 (lys23) rabbit mab

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Recombinant Rabbit Monoclonal Anti-Malonyl-Histone H3 (Lys23) Rabbit mAb Clone Number: B1555/65-H1L5 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K23ma Product Size 100μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K23ma UniProt ID P68431 Immunogen MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human ICC/IF 1:100 – 1:500 Human ChIP 6μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Malonylation of lysine is a newly identified reversible modification controlling protein activity. Sirt5, a regulatory enzyme for lysine malonylation, can catalyze lysine demalonylation reactions in vivo and in vitro. With integrated proteomic approaches and biochemistry analysis, lysine malonylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates. Lysine malonylation may provide a new paradigm in the cellular regulation, such as energy metabolism in diverse organisms. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): MCF-7, (+): MCF-7+ Sodium butyrate (50mM, 24hr) + Trichostatin A (500ngml, 4 hr) Protein loading amount: 20 μg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-):HeLa, (+):HeLa+Suramin butyrate(5mM,7h)+Sodium malonate(30mM,16h) Protein loading amount: 20 μg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa sodium salt(40µM,7h)+Sodium Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): C2C12, (+): C2C12+Suramin sodium salt(40µM,7h)+Sodium butyrate(5mM,7h)+Sodium malonate(30mM,16h) Protein loading amount: 20 μg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:5000 Primary ab incubation condition: 2 hours at room temperature Lysate: (-): BRL, (+): BRL + Suramin sodium (40µM, 7h) + Sodium butyrate (5mM, 7h) + Sodium malonate (30mM, 16h) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa ChIP Cell type: MCF-7+Sodium butyrate (50 mM, 24 hr)+trichostatin A (500 ng/ml, 4 hr) Cross-linking conditions: No cross-linking Amount of chromatin per IP: 5×10 6 cells Amount of Ab per IP: 6 μg Beads type and amount per IP: 50 μL of Protein A MagBeads Comment: The ChIP was performed with 6 μg of normal Rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH CDS region, RPL30, LDHA-promoter, LDHA and TuBBP10. Data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon. ICC/IF Cell line: HeLa+ +Suramin sodium salt (40µM,7h)+Sodium butyrate (5mM,7h)+Sodium malonate (30mM,16h) Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:500 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color red is the positive signal for :
Recombinant Rabbit Monoclonal Anti-Malonyl-Histone H3 (Lys23) Rabbit mAb Clone Number: B1555/65-H1L5 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K23ma Product Size 100μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K23ma UniProt ID P68431 Immunogen MW (kDa) 15 Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human ICC/IF 1:100 – 1:500 Human ChIP 6μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Malonylation of lysine is a newly identified reversible modification controlling protein activity. Sirt5, a regulatory enzyme for lysine malonylation, can catalyze lysine demalonylation reactions in vivo and in vitro. With integrated proteomic approaches and biochemistry analysis, lysine malonylation has been well demonstrated in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates. Lysine malonylation may provide a new paradigm in the cellular regulation, such as energy metabolism in diverse organisms. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): MCF-7, (+): MCF-7+ Sodium butyrate (50mM, 24hr) + Trichostatin A (500ngml, 4 hr) Protein loading amount: 20 μg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-):HeLa, (+):HeLa+Suramin butyrate(5mM,7h)+Sodium malonate(30mM,16h) Protein loading amount: 20 μg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa sodium salt(40µM,7h)+Sodium Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): C2C12, (+): C2C12+Suramin sodium salt(40µM,7h)+Sodium butyrate(5mM,7h)+Sodium malonate(30mM,16h) Protein loading amount: 20 μg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:5000 Primary ab incubation condition: 2 hours at room temperature Lysate: (-): BRL, (+): BRL + Suramin sodium (40µM, 7h) + Sodium butyrate (5mM, 7h) + Sodium malonate (30mM, 16h) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa ChIP Cell type: MCF-7+Sodium butyrate (50 mM, 24 hr)+trichostatin A (500 ng/ml, 4 hr) Cross-linking conditions: No cross-linking Amount of chromatin per IP: 5×10 6 cells Amount of Ab per IP: 6 μg Beads type and amount per IP: 50 μL of Protein A MagBeads Comment: The ChIP was performed with 6 μg of normal Rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH CDS region, RPL30, LDHA-promoter, LDHA and TuBBP10. Data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon. ICC/IF Cell line: HeLa+ +Suramin sodium salt (40µM,7h)+Sodium butyrate (5mM,7h)+Sodium malonate (30mM,16h) Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:500 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color red is the positive signal for