anti-lrp6 rabbit mab

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Recombinant Rabbit Monoclonal Anti-LRP6 Rabbit mAb Clone Number: JRMR-10493-174 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: Low-density lipoprotein receptor-related protein 6, LRP-6 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Low-density lipoprotein receptor-related protein 6, LRP-6 UniProt ID O75581 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background LRP6 is a single-pass transmembrane protein belonging to the low-density lipoprotein receptor (LDLR)-related protein family. Unlike other members of the LDLR family, LRP6 has four EGF and three LDLR repeats in the extracellular domain, and proline-rich motifs in the cytoplasmic domain. It functions as co-receptors for Wnt and is required for the canonical Wnt/β-catenin signaling pathway. LRP6 is highly homologous and has redundant roles during development. The activity of LRP6 can be inhibited by the binding of some members of the Dickkopf (DKK) family of proteins. Upon stimulation with Wnt, LRP6 is phosphorylated at multiple sites including Thr1479, Ser1490, and Thr1493 by kinases such as GSK-3 and CK1. Phosphorylated LRP6 recruits axin to the membrane and presumably activates β-catenin signaling. Cellular location Endoplasmic reticulum, Membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: HeLa, 2: HepG2 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 180 kDa Observed band size: 180 kDa :
Recombinant Rabbit Monoclonal Anti-LRP6 Rabbit mAb Clone Number: JRMR-10493-174 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: Low-density lipoprotein receptor-related protein 6, LRP-6 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Low-density lipoprotein receptor-related protein 6, LRP-6 UniProt ID O75581 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background LRP6 is a single-pass transmembrane protein belonging to the low-density lipoprotein receptor (LDLR)-related protein family. Unlike other members of the LDLR family, LRP6 has four EGF and three LDLR repeats in the extracellular domain, and proline-rich motifs in the cytoplasmic domain. It functions as co-receptors for Wnt and is required for the canonical Wnt/β-catenin signaling pathway. LRP6 is highly homologous and has redundant roles during development. The activity of LRP6 can be inhibited by the binding of some members of the Dickkopf (DKK) family of proteins. Upon stimulation with Wnt, LRP6 is phosphorylated at multiple sites including Thr1479, Ser1490, and Thr1493 by kinases such as GSK-3 and CK1. Phosphorylated LRP6 recruits axin to the membrane and presumably activates β-catenin signaling. Cellular location Endoplasmic reticulum, Membrane Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L(HRP Conjugate) Lysate: 1: HeLa, 2: HepG2 Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 180 kDa Observed band size: 180 kDa