anti-ldhb mouse mab

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Recombinant Anti-LDHB Mouse mAb Clone Number: 11965 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Rat Synonyms: LDH-B, L-lactate dehydrogenase B chain Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms LDH-B, L-lactate dehydrogenase B chain UniProt ID P07195 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:5000-1:20000 Human, Rat ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway.It is glycolytic enzymes and it is positively regulated by mTOR. Cellular location Cytoplasm, Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:20000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: HeLa, SH-SY5YProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 35 kDaObserved band size: 35 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: Jurkat, Rat brainProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 35 kDaObserved band size: 35 kDa ICC/IF Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with :
Recombinant Anti-LDHB Mouse mAb Clone Number: 11965 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Rat Synonyms: LDH-B, L-lactate dehydrogenase B chain Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms LDH-B, L-lactate dehydrogenase B chain UniProt ID P07195 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:5000-1:20000 Human, Rat ICC/IF 1:50 Human Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway.It is glycolytic enzymes and it is positively regulated by mTOR. Cellular location Cytoplasm, Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:20000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: HeLa, SH-SY5YProtein loading quantity: 20 μgExposure time: 10 sPredicted band size: 35 kDaObserved band size: 35 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:5000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate)Lysate: Jurkat, Rat brainProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 35 kDaObserved band size: 35 kDa ICC/IF Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with