anti-lc3b rabbit mab

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Recombinant Rabbit Monoclonal Anti-LC3B Rabbit mAb Clone Number: 10723 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: MAP1A/MAP1B LC3B, MAP1LC3B, MAP1ALC3 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms MAP1A/MAP1B LC3B, MAP1LC3B, MAP1ALC3 UniProt ID Q9GZQ8 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Microtubule-associated proteins 1A/1B light chain 3B (hereafter referred to as LC3B ) is a protein that is encoded by the MAP1LC3B gene in human. LC3B is a central protein in the autophagy pathway where it functions in autophagy substrate selection and autophagosome biogenesis. LC3B is a member of the highly conserved ATG8 protein family. ATG8 proteins are present in all known eukaryotic organisms. Newly synthesized LC3’s C-terminus is hydrolyzed by a cysteine protease called ATG4B exposing Gly120, termed LC3-I. LC3-I, through a series of ubiquitin-like reactions involving enzymes ATG7, ATG3, and ATG12-ATG5-ATG16, becomes conjugated to the head group of the lipid phosphatidylethanolamine. The lipid modified form of LC3, referred to as LC3-II, is believed to be involved in autophagosome membrane expansion and fusion events. Cellular location Cytoplasm, Membrane, Microtubule Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: C6, 2: Mouse brain, 3: HCT 116, 4: Saos-2Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 14, 16 kDa IHC-P Tissue: Human cerebrumSection type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-LC3B Rabbit mAb Clone Number: 10723 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: MAP1A/MAP1B LC3B, MAP1LC3B, MAP1ALC3 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms MAP1A/MAP1B LC3B, MAP1LC3B, MAP1ALC3 UniProt ID Q9GZQ8 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Microtubule-associated proteins 1A/1B light chain 3B (hereafter referred to as LC3B ) is a protein that is encoded by the MAP1LC3B gene in human. LC3B is a central protein in the autophagy pathway where it functions in autophagy substrate selection and autophagosome biogenesis. LC3B is a member of the highly conserved ATG8 protein family. ATG8 proteins are present in all known eukaryotic organisms. Newly synthesized LC3’s C-terminus is hydrolyzed by a cysteine protease called ATG4B exposing Gly120, termed LC3-I. LC3-I, through a series of ubiquitin-like reactions involving enzymes ATG7, ATG3, and ATG12-ATG5-ATG16, becomes conjugated to the head group of the lipid phosphatidylethanolamine. The lipid modified form of LC3, referred to as LC3-II, is believed to be involved in autophagosome membrane expansion and fusion events. Cellular location Cytoplasm, Membrane, Microtubule Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: C6, 2: Mouse brain, 3: HCT 116, 4: Saos-2Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 14 kDaObserved band size: 14, 16 kDa IHC-P Tissue: Human cerebrumSection type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with