anti-l-lactyllysine rabbit pab

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Recombinant Rabbit Monoclonal Anti-L-Lactyllysine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB FC Reactivity: All Synonyms: Kla Product Size 100μl OUT OF STOCK Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kla UniProt ID / Immunogen MW (kDa) Multiple Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:1000 All FC 1:50 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation,ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases.Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification,derived from the cellular metabolite lactate. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Peptide amount: 4 ng, 20 ng, 100 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-) HeLa; (+) HeLa+ sodium lactate (100 mM, 24 h)Protein loading amount: 20 μgExposure time: 60 sPredicted MW: Multiple kDaObserved MW: Multiple kDa Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: Mouse kidney, Mouse spleenProtein loading amount: 20 μgExposure time: 30 sPredicted MW: Multiple kDaObserved MW: Multiple kDa Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: HeLa, N2a, BRLProtein loading amount: 20 μgExposure time: 30 sPredicted MW: Multiple kDaObserved MW: Multiple kDa FC Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 0.1% Triton X-100Primary ab dilution: 1:50Secondary ab: Goat Anti-Rabbit IgGUnlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).Isotype control: Rabbit monoclonal IgG (Blue line).Comment: Line red is the positive signal for :
Recombinant Rabbit Monoclonal Anti-L-Lactyllysine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB FC Reactivity: All Synonyms: Kla Product Size 100μl OUT OF STOCK Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kla UniProt ID / Immunogen MW (kDa) Multiple Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500-1:1000 All FC 1:50 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation,ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases.Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification,derived from the cellular metabolite lactate. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Peptide amount: 4 ng, 20 ng, 100 ngExposure time: 60 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-) HeLa; (+) HeLa+ sodium lactate (100 mM, 24 h)Protein loading amount: 20 μgExposure time: 60 sPredicted MW: Multiple kDaObserved MW: Multiple kDa Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: Mouse kidney, Mouse spleenProtein loading amount: 20 μgExposure time: 30 sPredicted MW: Multiple kDaObserved MW: Multiple kDa Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:1000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: HeLa, N2a, BRLProtein loading amount: 20 μgExposure time: 30 sPredicted MW: Multiple kDaObserved MW: Multiple kDa FC Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 0.1% Triton X-100Primary ab dilution: 1:50Secondary ab: Goat Anti-Rabbit IgGUnlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).Isotype control: Rabbit monoclonal IgG (Blue line).Comment: Line red is the positive signal for