anti-l-lactyl-histone h3 (lys27) rabbit pab

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Anti-L-Lactyl-Histone H3 (Lys27) Rabbit pAb Clone Number: W1664-FT3 Host: Rabbit Clonality: Polyclonal Applications： WB ChIP Reactivity: Human, Mouse Synonyms: H3K27la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K27la UniProt ID P68431 Immunogen L-lactylated human histone H3 (Lys27) peptide MW (kDa) 15 Specificity This antibody detects histone H3 only when it is lactylated at Lys27, but not the H3 peptide acetylated,butyrylated, crotonylated, 2-hydroxybutyrylated, β-hydroxybutyrylated and the unmodified at Lys27, and not the H3 peptide acetylated,lactylated, crotonylated, 2-hydroxybutyrylated and β-hydroxybutyrylated at Lys9. Product Usage Information Applications Dilution Recommended Species WB 1:2000 Human, Mouse ChIP 6 μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone acetylation occurs primarily at multiple lysine residues on the aminoterminal of core histones, in response to various stimuli and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Mostly, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are major regulating factors. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-rabbit IgG H&L (HRP) Peptide amount: 1 ng, 4 ng, 16 ng, 64ng Exposure time: 10 s The list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): Jurkat, (+): Jurkat + Sodium Lactate (25 mM, 24hr)Protein loading amount: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): HeLa, (+): HeLa + Sodium Lactate (25 mM, 24hr)Protein loading amount: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): Neuro-2a, (+): Neuro-2a + Sodium Lactate (25 mM, 24hr)Protein loading amount: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa ChIP Cell type: HeLa+Lac Na(25mM, 24h)(-)/(+) Cross-linking conditions: No cross-linking. Amount of chromatin per IP: 2×106 cells Amount of Ab per IP: 6 μg Beads type and amount per IP: 50 μL of Protein A MagBeads Comment: The ChIP was performed with 6 μg of normal Rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH-CDS and LDHA-CDS. Data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon. :
Anti-L-Lactyl-Histone H3 (Lys27) Rabbit pAb Clone Number: W1664-FT3 Host: Rabbit Clonality: Polyclonal Applications： WB ChIP Reactivity: Human, Mouse Synonyms: H3K27la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K27la UniProt ID P68431 Immunogen L-lactylated human histone H3 (Lys27) peptide MW (kDa) 15 Specificity This antibody detects histone H3 only when it is lactylated at Lys27, but not the H3 peptide acetylated,butyrylated, crotonylated, 2-hydroxybutyrylated, β-hydroxybutyrylated and the unmodified at Lys27, and not the H3 peptide acetylated,lactylated, crotonylated, 2-hydroxybutyrylated and β-hydroxybutyrylated at Lys9. Product Usage Information Applications Dilution Recommended Species WB 1:2000 Human, Mouse ChIP 6 μg per 5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone acetylation occurs primarily at multiple lysine residues on the aminoterminal of core histones, in response to various stimuli and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Mostly, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are major regulating factors. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-rabbit IgG H&L (HRP) Peptide amount: 1 ng, 4 ng, 16 ng, 64ng Exposure time: 10 s The list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): Jurkat, (+): Jurkat + Sodium Lactate (25 mM, 24hr)Protein loading amount: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): HeLa, (+): HeLa + Sodium Lactate (25 mM, 24hr)Protein loading amount: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): Neuro-2a, (+): Neuro-2a + Sodium Lactate (25 mM, 24hr)Protein loading amount: 20 μgExposure time: 60 sPredicted MW: 15 kDaObserved MW: 15 kDa ChIP Cell type: HeLa+Lac Na(25mM, 24h)(-)/(+) Cross-linking conditions: No cross-linking. Amount of chromatin per IP: 2×106 cells Amount of Ab per IP: 6 μg Beads type and amount per IP: 50 μL of Protein A MagBeads Comment: The ChIP was performed with 6 μg of normal Rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH-CDS and LDHA-CDS. Data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.