anti-l-lactyl-histone h3 (lys14) rabbit mab

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Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H3 (Lys14) Rabbit mAb Clone Number: PA-111-9 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K14la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K14la UniProt ID P68431 Immunogen L-lactylated human histone H3 (Lys14) peptide MW (kDa) 15 Specificity Anti-L-Lactyl-Histone H3 (Lys14) Rabbit mAb detects endogenous levels of histone H3 only when it is lactylated at Lys14. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ICC/IF 1:50 Human ChIP 6 μg/5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases. Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification, derived from the cellular metabolite lactate. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Peptide amount: 1ng, 4 ng, 16 ng, 64 ngExposure time: 30 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HeLa; (+): HeLa+ sodium lactate (100 mM, 24 hours)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 15 kDaObserved band size: 15 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Neuro-2a, 2: BRL, 3: Rat kidney, 4: Mouse kidney, 5: Recombinant histone H3 (20 ng)Protein loading amount: 20 μg Exposure time: 60 sPredicted band size: 15 kDaObserved band size: 15 kDa ICC/IF Cell line: (A) HeLa, (B) HeLa+ sodium lactate (100mM, 24hr) Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:100 Primary incubation condition: 4℃ overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Description: Color green is the positive signal for :
Recombinant Rabbit Monoclonal Anti-L-Lactyl-Histone H3 (Lys14) Rabbit mAb Clone Number: PA-111-9 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF ChIP Reactivity: Human, Mouse, Rat Synonyms: H3K14la Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H3K14la UniProt ID P68431 Immunogen L-lactylated human histone H3 (Lys14) peptide MW (kDa) 15 Specificity Anti-L-Lactyl-Histone H3 (Lys14) Rabbit mAb detects endogenous levels of histone H3 only when it is lactylated at Lys14. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat ICC/IF 1:50 Human ChIP 6 μg/5×106 cells Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are subject to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases. Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification, derived from the cellular metabolite lactate. Cellular location Nucleus Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Peptide amount: 1ng, 4 ng, 16 ng, 64 ngExposure time: 30 sThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: (-): HeLa; (+): HeLa+ sodium lactate (100 mM, 24 hours)Protein loading amount: 20 μgExposure time: 60 sPredicted band size: 15 kDaObserved band size: 15 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: Neuro-2a, 2: BRL, 3: Rat kidney, 4: Mouse kidney, 5: Recombinant histone H3 (20 ng)Protein loading amount: 20 μg Exposure time: 60 sPredicted band size: 15 kDaObserved band size: 15 kDa ICC/IF Cell line: (A) HeLa, (B) HeLa+ sodium lactate (100mM, 24hr) Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:100 Primary incubation condition: 4℃ overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Description: Color green is the positive signal for