anti-acetylmethionine rabbit pab

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Anti-Acetylmethionine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ICC/IF FC Reactivity: Human, Mouse, Rat, A.thaliana, S.cerevisiae Synonyms: Mac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Mac UniProt ID / Immunogen Acetylated methionine peptides MW (kDa) Multiple Specificity Anti-Acetylmethionine Rabbit pAb detects protein N-terminal acetylation on methionine residues. This pan antibody recognizes acetylated methionine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat, Arabidopsis, S. cerevisiae ICC/IF 1:50 Human FC 1:50 – 1:100 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Protein N-terminal acetylation (Nt-acetylation) is one of the most common modifications in eukaryotic organisms. Nt-acetylation, in contrast to lysine acetylation, refers to the transfer of an acetyl (Ac-) group to the N terminus of a protein, i.e., the amino group of the first methionine in proteins. Nt-acetylation is carried out by N-terminal acetyl-transferases (NATs) using acetyl coenzyme A (Ac-CoA) as the donor of the Ac group. Protein-protein interactions are in some cases mediated or strengthened by Nt-acetylation. Nt-acetylation can also promote membrane targeting of certain proteins, through either interaction with an integral membrane protein or direct bundling with membrane lipids. Specifically, Nt-acetylation plays a critical role in controlling autophagy by finetuning multiple steps in the process. Cellular location Nucleus, Cytoplasm Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Peptide amount: 1: 4ng, 20 ng, 100 ng; 2-8: 100 ng Exposure time: 30 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: HeLa, 2: NIH/3T3, 3: BRL, 4: Arabidopsis, 5: S. cerevisiaeProtein loading amount: 20 μgExposure time: 60 secondsPredicted band size: MultipleObserved band size: Multiple ICC/IF Cell line: HeLa Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (blue) Description: The green color represents the positive signal observed with :
Anti-Acetylmethionine Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB ICC/IF FC Reactivity: Human, Mouse, Rat, A.thaliana, S.cerevisiae Synonyms: Mac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Mac UniProt ID / Immunogen Acetylated methionine peptides MW (kDa) Multiple Specificity Anti-Acetylmethionine Rabbit pAb detects protein N-terminal acetylation on methionine residues. This pan antibody recognizes acetylated methionine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse, Rat, Arabidopsis, S. cerevisiae ICC/IF 1:50 Human FC 1:50 – 1:100 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Protein N-terminal acetylation (Nt-acetylation) is one of the most common modifications in eukaryotic organisms. Nt-acetylation, in contrast to lysine acetylation, refers to the transfer of an acetyl (Ac-) group to the N terminus of a protein, i.e., the amino group of the first methionine in proteins. Nt-acetylation is carried out by N-terminal acetyl-transferases (NATs) using acetyl coenzyme A (Ac-CoA) as the donor of the Ac group. Protein-protein interactions are in some cases mediated or strengthened by Nt-acetylation. Nt-acetylation can also promote membrane targeting of certain proteins, through either interaction with an integral membrane protein or direct bundling with membrane lipids. Specifically, Nt-acetylation plays a critical role in controlling autophagy by finetuning multiple steps in the process. Cellular location Nucleus, Cytoplasm Images Dot Blot Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Peptide amount: 1: 4ng, 20 ng, 100 ng; 2-8: 100 ng Exposure time: 30 secondsThe list of peptides is included in the table below. WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: HeLa, 2: NIH/3T3, 3: BRL, 4: Arabidopsis, 5: S. cerevisiaeProtein loading amount: 20 μgExposure time: 60 secondsPredicted band size: MultipleObserved band size: Multiple ICC/IF Cell line: HeLa Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (blue) Description: The green color represents the positive signal observed with