anti-itk mouse mab

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Recombinant Anti-ITK Mouse mAb Clone Number: JMMR-3569 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: Tyrosine-protein kinase, TSK, EMT, LYK Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Tyrosine-protein kinase, TSK, EMT, LYK UniProt ID Q08881 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Interleukin-2 inducible T cell kinase (ITK, Emt or Tsk) is a member of the non-receptor protein tyrosine kinases. Family members of Itk include Tec, Btk, Rlk, and Bmx and are all defined by a common structure: an amino-terminal PH domain, a Tec-homology domain, and an SH3 and SH2 domain followed by a carboxy-terminal kinase domain. Tec, Rlk, and ITK are expressed in T cells and activated in response to T cell receptor (TCR) engagement. Data demonstrate that ITK functions in signal transduction downstream of TCR and activates PLCgamma1 and Erk. Lck directly activates ITK through phosphorylation in the conserved activation loop at Tyr511, and furthermore, ITK is autophosphorylated in the SH3 domain at Tyr180. ITK-Y180F is still capable of phosphorylating PLCgamma1 in contrast to ITK-Y511F, which has lost that function. Itk -/- mice show reduced lung inflammation, eosinophil infiltration, and mucous production in response to allergic asthma induction. Thus, ITK could become a desirable target for anti-asthmatic treatments. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: Raji (Negative control); 2: JurKat; 3: MOLT-4; 4: HuT-78 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 72 kDa Observed band size: 70 kDa :
Recombinant Anti-ITK Mouse mAb Clone Number: JMMR-3569 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: Tyrosine-protein kinase, TSK, EMT, LYK Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms Tyrosine-protein kinase, TSK, EMT, LYK UniProt ID Q08881 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Interleukin-2 inducible T cell kinase (ITK, Emt or Tsk) is a member of the non-receptor protein tyrosine kinases. Family members of Itk include Tec, Btk, Rlk, and Bmx and are all defined by a common structure: an amino-terminal PH domain, a Tec-homology domain, and an SH3 and SH2 domain followed by a carboxy-terminal kinase domain. Tec, Rlk, and ITK are expressed in T cells and activated in response to T cell receptor (TCR) engagement. Data demonstrate that ITK functions in signal transduction downstream of TCR and activates PLCgamma1 and Erk. Lck directly activates ITK through phosphorylation in the conserved activation loop at Tyr511, and furthermore, ITK is autophosphorylated in the SH3 domain at Tyr180. ITK-Y180F is still capable of phosphorylating PLCgamma1 in contrast to ITK-Y511F, which has lost that function. Itk -/- mice show reduced lung inflammation, eosinophil infiltration, and mucous production in response to allergic asthma induction. Thus, ITK could become a desirable target for anti-asthmatic treatments. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: Raji (Negative control); 2: JurKat; 3: MOLT-4; 4: HuT-78 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 72 kDa Observed band size: 70 kDa