anti-irf8 mouse mab

Vapor Pressure :
Recombinant Anti-IRF8 Mouse mAb Clone Number: JMMR-2709 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: H-ICSBP, ICSBP, ICSBP1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms H-ICSBP, ICSBP, ICSBP1 UniProt ID Q02556 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:4000 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background IRF-8 is a member of Interferon regulatory factors family, which is expressed predominately in hematopoietic cells and is further increased upon treatment with interferon. IRF-8 can function as a transcription repressor of ICS-containing promoters. Expression of IRF-8 can lead to the downregulation of the anti-apoptotic protein Bcl-2. Originally described as being induced by IFN-γ, IRF-8 expression is also elevated by IRF-α as well as IL-12 in NK and T cells. IRF-8 deficient mice have enhanced susceptibility to various pathogens and impaired production of interferons, as well as deregulated hematopoiesis that resembles chronic myelogenous leukemia. IRF-8 also regulates bone metabolism by suppressing osteoclast formation. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:4000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: THP-1, 2: Daudi, 3: Ramos Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 48 kDa Observed band size: 48 kDa ICC/IF Cell line: THP-1 Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with :
Recombinant Anti-IRF8 Mouse mAb Clone Number: JMMR-2709 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human Synonyms: H-ICSBP, ICSBP, ICSBP1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2b/kappa Conjugate Unconjugated Synonyms H-ICSBP, ICSBP, ICSBP1 UniProt ID Q02556 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:4000 Human ICC/IF 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background IRF-8 is a member of Interferon regulatory factors family, which is expressed predominately in hematopoietic cells and is further increased upon treatment with interferon. IRF-8 can function as a transcription repressor of ICS-containing promoters. Expression of IRF-8 can lead to the downregulation of the anti-apoptotic protein Bcl-2. Originally described as being induced by IFN-γ, IRF-8 expression is also elevated by IRF-α as well as IL-12 in NK and T cells. IRF-8 deficient mice have enhanced susceptibility to various pathogens and impaired production of interferons, as well as deregulated hematopoiesis that resembles chronic myelogenous leukemia. IRF-8 also regulates bone metabolism by suppressing osteoclast formation. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:4000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgGH&L(HRP Conjugate) Lysate: 1: THP-1, 2: Daudi, 3: Ramos Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 48 kDa Observed band size: 48 kDa ICC/IF Cell line: THP-1 Fixative: 4% Paraformaldehyde Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with