anti-acetyllysine rabbit pab (bsa and azide free)

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Anti-Acetyllysine Rabbit pAb (BSA and Azide Free) Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB FC Reactivity: All Synonyms: Kac Product Size 100 μg Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kac UniProt ID / Immunogen Acetylated lysine peptides MW (kDa) Multiple Specificity Anti-Acetyllysine Rabbit pAb (BSA and Azide Free) detects proteins post-translationally modified by Acetylation on lysine residues. This pan antibody recognizes Acetylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:1000 All FC 1:100-1:300 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS Storage Before reconstitution, centrifuge the vial at 12,000 x g for 20 seconds. Add sterile, deionized water to achieve a final antibody concentration of 1 mg/ml. The antibody is now in 1X PBS solution. Aliquot and store at -80°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone acetylation occurs primarily at multiple lysine residues on the amino-terminal of core histones, in response to various stimuli and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Mostly, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are major regulating factors. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Immunogen peptide quantity: 4 ng, 20 ng, 100 ngExposure time: 60 sThe list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4℃ overnightSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-): HeLa, (+): HeLa+ Sodium Butyrate (30mM, 4hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: Multiple kDaObserved MW: Multiple kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4℃ overnightSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-): MCF-7, (+): MCF-7+ Sodium Butyrate (5mM, 24hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: Multiple kDaObserved MW: Multiple kDa FC Cell line: HeLaFixation: 4% ParaformaldehydePermeabilization: 90% MethanolPrimary Ab dilution: 1:300Secondary Ab: Goat Anti-Rabbit IgGUnlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).Isotype control: Rabbit monoclonal IgG (Blue line).Description: Line red is the positive signal for :
Anti-Acetyllysine Rabbit pAb (BSA and Azide Free) Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB FC Reactivity: All Synonyms: Kac Product Size 100 μg Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kac UniProt ID / Immunogen Acetylated lysine peptides MW (kDa) Multiple Specificity Anti-Acetyllysine Rabbit pAb (BSA and Azide Free) detects proteins post-translationally modified by Acetylation on lysine residues. This pan antibody recognizes Acetylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:1000 All FC 1:100-1:300 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS Storage Before reconstitution, centrifuge the vial at 12,000 x g for 20 seconds. Add sterile, deionized water to achieve a final antibody concentration of 1 mg/ml. The antibody is now in 1X PBS solution. Aliquot and store at -80°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone acetylation occurs primarily at multiple lysine residues on the amino-terminal of core histones, in response to various stimuli and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Mostly, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16, and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56, and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are major regulating factors. Cellular location / Images Dot Blot Blocking buffer: 5% NFDM/TBSTPrimary ab dilution: 1:2000Primary ab incubation condition: 2 hours at room temperatureSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)Immunogen peptide quantity: 4 ng, 20 ng, 100 ngExposure time: 60 sThe list of peptides is included in the table below: WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4℃ overnightSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-): HeLa, (+): HeLa+ Sodium Butyrate (30mM, 4hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: Multiple kDaObserved MW: Multiple kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4℃ overnightSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)Lysate: (-): MCF-7, (+): MCF-7+ Sodium Butyrate (5mM, 24hr)Protein loading quantity: 20 μgExposure time: 60 sPredicted MW: Multiple kDaObserved MW: Multiple kDa FC Cell line: HeLaFixation: 4% ParaformaldehydePermeabilization: 90% MethanolPrimary Ab dilution: 1:300Secondary Ab: Goat Anti-Rabbit IgGUnlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).Isotype control: Rabbit monoclonal IgG (Blue line).Description: Line red is the positive signal for