anti-inos mouse mab

Vapor Pressure :
Recombinant Anti-iNOS Mouse mAb Clone Number: 13451 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Mouse Synonyms: HEP-NOS, NOS2, NOS2A Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms HEP-NOS, NOS2, NOS2A UniProt ID P35228 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Mouse ICC/IF 1:50 Mouse Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background iNOS is a Nitric Oxide Synthase (NOS) that catalyzes the formation of nitric oxide (NO) and citrulline from L-arginine, oxygen, and cofactors.Nitric oxide catalyzed by iNOS is involved in host defense against protozoa, bacteria, fungi and viruses. Unlike constitutively expressed eNOS and nNos, iNOS is not usually expressed in quiescent cells. iNOS is transcriptionally induced in response to bacterial endotoxins, such as LPS and proinflammatory cytokines, in macrophages and various other cell types. Transcription factors involved in iNOS transcription include NF-κB, AP-1 and STAT. Different signaling pathways either promote (Jak1/2, PKC, c-Raf, p38 MAP kinase and p44/42 MAP kinase) or inhibit (PI3 kinase) iNOS expression depending on stimulus and cell type. Cellular location Cytosol Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: RAW 264.7, 2: RAW 264.7+LPS (1 μg/mL, 6h) Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 131 kDa Observed band size: 130 kDa ICC/IF Cell line: (A) RAW 264.7, (B) RAW 264.7+LPS (0.1 mg/mL, 6h) Fixative: 100% Ice-cold methanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with :
Recombinant Anti-iNOS Mouse mAb Clone Number: 13451 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Mouse Synonyms: HEP-NOS, NOS2, NOS2A Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG1/kappa Conjugate Unconjugated Synonyms HEP-NOS, NOS2, NOS2A UniProt ID P35228 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Mouse ICC/IF 1:50 Mouse Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background iNOS is a Nitric Oxide Synthase (NOS) that catalyzes the formation of nitric oxide (NO) and citrulline from L-arginine, oxygen, and cofactors.Nitric oxide catalyzed by iNOS is involved in host defense against protozoa, bacteria, fungi and viruses. Unlike constitutively expressed eNOS and nNos, iNOS is not usually expressed in quiescent cells. iNOS is transcriptionally induced in response to bacterial endotoxins, such as LPS and proinflammatory cytokines, in macrophages and various other cell types. Transcription factors involved in iNOS transcription include NF-κB, AP-1 and STAT. Different signaling pathways either promote (Jak1/2, PKC, c-Raf, p38 MAP kinase and p44/42 MAP kinase) or inhibit (PI3 kinase) iNOS expression depending on stimulus and cell type. Cellular location Cytosol Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Lysate: 1: RAW 264.7, 2: RAW 264.7+LPS (1 μg/mL, 6h) Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 131 kDa Observed band size: 130 kDa ICC/IF Cell line: (A) RAW 264.7, (B) RAW 264.7+LPS (0.1 mg/mL, 6h) Fixative: 100% Ice-cold methanol Permeabilization: 0.1% Triton X-100 Primary Ab dilution: 1:50 Primary Ab incubation condition: 4°C overnight Secondary Ab: Goat Anti-Mouse IgGNuclear counter stain: DAPI (Blue) Description: The green color represents the positive signal observed with