anti-acetyllysine rabbit mab

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Recombinant Rabbit Monoclonal Anti-Acetyllysine Rabbit mAb Clone Number: PA-070-53 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P IP ChIP Reactivity: All Synonyms: Kac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kac UniProt ID / Immunogen Acetylated lysine peptides MW (kDa) Multiple Specificity Anti-Acetyllysine Rabbit mAb detects proteins with acetylated lysine residues. This pan antibody recognizes acetylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All IHC-P 1:200 – 1:1000 Human, Mouse, Rat IP 1:50 All ChIP 6 μg per 5×106 cells All Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics gene activities, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The mis-regulated protein acetylation status has been implicated in cancers and other diseases, and HDACs have become likely targets for anti-cancer drugs. Cellular location / Images Dot Blot Peptide amount: 4 ng, 20ng, 100 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Exposure time: 30 secondsThe list of peptides used in the experiment is provided in the table below. WB Lysate: HeLaProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab: Lane 1, :
Recombinant Rabbit Monoclonal Anti-Acetyllysine Rabbit mAb Clone Number: PA-070-53 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P IP ChIP Reactivity: All Synonyms: Kac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kac UniProt ID / Immunogen Acetylated lysine peptides MW (kDa) Multiple Specificity Anti-Acetyllysine Rabbit mAb detects proteins with acetylated lysine residues. This pan antibody recognizes acetylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All IHC-P 1:200 – 1:1000 Human, Mouse, Rat IP 1:50 All ChIP 6 μg per 5×106 cells All Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics gene activities, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. The mis-regulated protein acetylation status has been implicated in cancers and other diseases, and HDACs have become likely targets for anti-cancer drugs. Cellular location / Images Dot Blot Peptide amount: 4 ng, 20ng, 100 ng Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Exposure time: 30 secondsThe list of peptides used in the experiment is provided in the table below. WB Lysate: HeLaProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab: Lane 1,