anti-il-1 beta rabbit mab

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Recombinant Rabbit Monoclonal Anti-IL-1 beta Rabbit mAb Clone Number: JRMR-10718-99B Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: Interleukin-1 beta, Catabolin, IL1B, IL1F2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Interleukin-1 beta, Catabolin, IL1B, IL1F2 UniProt ID P01584 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses. It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form. Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity. Cellular location Cytoplasm, Secreted, Lysosome Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: (-): THP-1, (+): THP-1+TPA (12-O-Tetradecanoylphorbol-13-acetate) [80nM, overnight] + LPS (Lipopolysaccharide) [100ng/ml, 6 hr] +BFA (Brefeldin A) [300 ng/mL, last 3 hr of stimulation] Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 31 kDa Observed band size: 27-32 kDa :
Recombinant Rabbit Monoclonal Anti-IL-1 beta Rabbit mAb Clone Number: JRMR-10718-99B Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: Interleukin-1 beta, Catabolin, IL1B, IL1F2 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms Interleukin-1 beta, Catabolin, IL1B, IL1F2 UniProt ID P01584 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses. It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form. Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity. Cellular location Cytoplasm, Secreted, Lysosome Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: (-): THP-1, (+): THP-1+TPA (12-O-Tetradecanoylphorbol-13-acetate) [80nM, overnight] + LPS (Lipopolysaccharide) [100ng/ml, 6 hr] +BFA (Brefeldin A) [300 ng/mL, last 3 hr of stimulation] Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 31 kDa Observed band size: 27-32 kDa