anti-acetyllysine mouse mab

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Anti-Acetyllysine Mouse mAb Clone Number: Kac-01 Host: Mouse Clonality: Monoclonal Applications： WB IHC-P IP Reactivity: All Synonyms: Kac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kac UniProt ID / Immunogen Acetylated lysine peptides MW (kDa) Multiple Specificity Anti-Acetyllysine Mouse mAb detects proteins with acetylated lysine residues. This pan antibody recognizes acetylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All IHC-P 1:500 – 1:2000 Human, Mouse IP 1:25 – 1:300 Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The reversible lysine acetylation of histones and non-histone proteins is a crucial post-translational modification that regulates diverse cellular processes, including chromatin dynamics, gene expression, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 histone deacetylases (HDACs) have been identified, but the mechanisms governing substrate selection and site specificity of these enzymes remain unclear. Dysregulation of protein acetylation has been implicated in the development of cancers and other diseases, and HDACs have become likely targets for anti-cancer drugs. Cellular location / Images Dot Blot Protein amount: 4 ng, 20 ng, 100 ng, 500 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of proteins used in the experiment is provided below.Lane 1: Acetylated BSA. Lane 2: Propionylated BSA. Lane 3: Butyrylated BSA.Lane 4: Unmodified BSA. WB Lysates: HeLa cells Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: (A) :
Anti-Acetyllysine Mouse mAb Clone Number: Kac-01 Host: Mouse Clonality: Monoclonal Applications： WB IHC-P IP Reactivity: All Synonyms: Kac Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Kac UniProt ID / Immunogen Acetylated lysine peptides MW (kDa) Multiple Specificity Anti-Acetyllysine Mouse mAb detects proteins with acetylated lysine residues. This pan antibody recognizes acetylated lysine independent of its surrounding sequences. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 All IHC-P 1:500 – 1:2000 Human, Mouse IP 1:25 – 1:300 Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background The reversible lysine acetylation of histones and non-histone proteins is a crucial post-translational modification that regulates diverse cellular processes, including chromatin dynamics, gene expression, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 histone deacetylases (HDACs) have been identified, but the mechanisms governing substrate selection and site specificity of these enzymes remain unclear. Dysregulation of protein acetylation has been implicated in the development of cancers and other diseases, and HDACs have become likely targets for anti-cancer drugs. Cellular location / Images Dot Blot Protein amount: 4 ng, 20 ng, 100 ng, 500 ngBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds The list of proteins used in the experiment is provided below.Lane 1: Acetylated BSA. Lane 2: Propionylated BSA. Lane 3: Butyrylated BSA.Lane 4: Unmodified BSA. WB Lysates: HeLa cells Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: (A)