anti-ikk gamma rabbit mab

Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-IKK gamma Rabbit mAb Clone Number: JRMR-10993-27 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF FC IP Reactivity: Human, Mouse Synonyms: NEMO, IKKG, IKKAP1, FIP-3, IKBKG Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms NEMO, IKKG, IKKAP1, FIP-3, IKBKG UniProt ID Q9Y6K9 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human ICC/IF 1:50 Human, Mouse FC 1:50 – 1:100 Human IP 1:25 – 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background IKK gamma (IKKγ) is a subunit of a high molecular weight IκB kinase (IKK) complex, which is involved in NF-κB activation. The catalysis of IκB kinase (IKK) complex is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation. Activation of the NF-κB pathway by the T-cell lymphotrophic virus Tax protein or by TNF-α treatment leads to IKKβ-dependent phosphorylation of human IKKγ, primarily at Ser376. In mice, mutation of the orthologous residue (Ser369) to alanine leads to enhanced IKKγ-mediated stimulation of IKKβ kinase activity. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: K562, 3: JurkatProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 48 kDaObserved band size: 48 kDa ICC/IF Cell line: HeLaFixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Counter stain: Tubulin (Red)Description: The green color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-IKK gamma Rabbit mAb Clone Number: JRMR-10993-27 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF FC IP Reactivity: Human, Mouse Synonyms: NEMO, IKKG, IKKAP1, FIP-3, IKBKG Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms NEMO, IKKG, IKKAP1, FIP-3, IKBKG UniProt ID Q9Y6K9 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human ICC/IF 1:50 Human, Mouse FC 1:50 – 1:100 Human IP 1:25 – 1:50 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background IKK gamma (IKKγ) is a subunit of a high molecular weight IκB kinase (IKK) complex, which is involved in NF-κB activation. The catalysis of IκB kinase (IKK) complex is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation. Activation of the NF-κB pathway by the T-cell lymphotrophic virus Tax protein or by TNF-α treatment leads to IKKβ-dependent phosphorylation of human IKKγ, primarily at Ser376. In mice, mutation of the orthologous residue (Ser369) to alanine leads to enhanced IKKγ-mediated stimulation of IKKβ kinase activity. Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: K562, 3: JurkatProtein loading quantity: 20 μgExposure time: 60 sPredicted band size: 48 kDaObserved band size: 48 kDa ICC/IF Cell line: HeLaFixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100Primary Ab dilution: 1:50Primary Ab incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Counter stain: Tubulin (Red)Description: The green color represents the positive signal observed with