anti-ikk alpha rabbit mab

Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-IKK alpha Rabbit mAb Clone Number: JRMR-10700-48G Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: CHUK1, I-kappa-B kinase 1, IKK-A, IKK1, NFKBIKA, TCF-16 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CHUK1, I-kappa-B kinase 1, IKK-A, IKK1, NFKBIKA, TCF-16 UniProt ID O15111 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background IKKa is IkB Kinase alpha subunit. The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB (p50 and p65). The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. Upon its activation, the IKK complex phosphorylates IkBa at Ser32 and Ser36. This results ultimately in the degradation of IkB and the activation and nuclear translocation of NFkB. While both IKK-alpha and IKK-beta subunits are Ser/Thr kinases, IKK-beta appears to be the principal kinase, as IKKa is not required for activation of the IKK complex and the subsequent degradation of IkBa. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4℃ overnightSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: SK-OV-3, 2: HCT-116, 3: DaudiProtein loading amount: 20 μgExposure time: 60 secondsPredicted band size: 85 kDa Observed band size: 85 kDa :
Recombinant Rabbit Monoclonal Anti-IKK alpha Rabbit mAb Clone Number: JRMR-10700-48G Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: CHUK1, I-kappa-B kinase 1, IKK-A, IKK1, NFKBIKA, TCF-16 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CHUK1, I-kappa-B kinase 1, IKK-A, IKK1, NFKBIKA, TCF-16 UniProt ID O15111 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background IKKa is IkB Kinase alpha subunit. The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB (p50 and p65). The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. Upon its activation, the IKK complex phosphorylates IkBa at Ser32 and Ser36. This results ultimately in the degradation of IkB and the activation and nuclear translocation of NFkB. While both IKK-alpha and IKK-beta subunits are Ser/Thr kinases, IKK-beta appears to be the principal kinase, as IKKa is not required for activation of the IKK complex and the subsequent degradation of IkBa. Cellular location Nucleus, Cytoplasm Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 4℃ overnightSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: SK-OV-3, 2: HCT-116, 3: DaudiProtein loading amount: 20 μgExposure time: 60 secondsPredicted band size: 85 kDa Observed band size: 85 kDa