anti-ikk alpha rabbit mab

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Recombinant Rabbit Monoclonal Anti-IKK alpha Rabbit mAb Clone Number: 12125 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse Synonyms: IKKA, TCF16 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms IKKA, TCF16 UniProt ID O15111 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse ICC/IF 1:100 – 1:200 Human, Mouse Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF- Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HeLa, SH-SY5Y, A20, Raw264.7Protein loading quantity: 20 μgExposure time: 3 sPredicted band size: 85 kDaObserved band size: 85 kDa ICC/IF Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:200Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-IKK alpha Rabbit mAb Clone Number: 12125 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB ICC/IF Reactivity: Human, Mouse Synonyms: IKKA, TCF16 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms IKKA, TCF16 UniProt ID O15111 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse ICC/IF 1:100 – 1:200 Human, Mouse Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF- Cellular location Cytoplasm, Nucleus Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: HeLa, SH-SY5Y, A20, Raw264.7Protein loading quantity: 20 μgExposure time: 3 sPredicted band size: 85 kDaObserved band size: 85 kDa ICC/IF Cell line: HeLaFixative: 4% ParaformaldehydePermeabilization: 0.1% TritonX-100Primary Ab dilution: 1:200Primary incubation condition: 4°C overnightSecondary Ab: Goat Anti-Rabbit IgGNuclear counter stain: DAPI (Blue)Description: The green color represents the positive signal observed with