anti-acetyl-sod2 (lys68) rabbit mab

Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-Acetyl-SOD2 (Lys68) Rabbit mAb Clone Number: JMMR-3498 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: SOD2, Superoxide dismutase Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms SOD2, Superoxide dismutase UniProt ID P04179 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Rat IHC-P 1:50 – 1:100 Human, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Manganese superoxide dismutase (MnSOD), also named as SOD2, is a mitochondrial detoxification enzyme that catalyzes the conversion of superoxide to hydrogen peroxide. Hydrogen peroxide is then decomposed to water by catalase, glutathione peroxidase, or peroxiredoxins. MnSOD and other enzymes involved in antioxidant defense protect cells from reactive oxygen species (ROS). Calorie restriction leads to SIRT3-mediated deacetylation of MnSOD and the subsequent increase of its antioxidant activity. MnSOD also plays an essential role in mediating the protective effect of mTOR inhibition to reduce epithelial stem cell senescence. Cellular location Mitochondrion matrix Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: Human liver, 2: Rat kidney Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 25 kDa Observed band size: 20 kDa IHC-P Tissue: Human colon cancer Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-Acetyl-SOD2 (Lys68) Rabbit mAb Clone Number: JMMR-3498 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat Synonyms: SOD2, Superoxide dismutase Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG/kappa Conjugate Unconjugated Synonyms SOD2, Superoxide dismutase UniProt ID P04179 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Rat IHC-P 1:50 – 1:100 Human, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Manganese superoxide dismutase (MnSOD), also named as SOD2, is a mitochondrial detoxification enzyme that catalyzes the conversion of superoxide to hydrogen peroxide. Hydrogen peroxide is then decomposed to water by catalase, glutathione peroxidase, or peroxiredoxins. MnSOD and other enzymes involved in antioxidant defense protect cells from reactive oxygen species (ROS). Calorie restriction leads to SIRT3-mediated deacetylation of MnSOD and the subsequent increase of its antioxidant activity. MnSOD also plays an essential role in mediating the protective effect of mTOR inhibition to reduce epithelial stem cell senescence. Cellular location Mitochondrion matrix Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: Human liver, 2: Rat kidney Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 25 kDa Observed band size: 20 kDa IHC-P Tissue: Human colon cancer Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with