anti-hsp90 beta rabbit mab

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Recombinant Rabbit Monoclonal Anti-HSP90 beta Rabbit mAb Clone Number: JRMR-10306-32 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P IP Reactivity: Human, Mouse, Rat Synonyms: HSP 90, HSP90AB1, HSP90B, HSPC2, HSPCB Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms HSP 90, HSP90AB1, HSP90B, HSPC2, HSPCB UniProt ID P08238 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:10000-1:50000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Hsp90 (90 kDa heat shock protein) is a molecular chaperone that aids protein folding and quality control for a large number of ‘client’ proteins. Hsp90 operates as dimer and has intrinsic ATPase activity. The Hsp90 dimer acts in concert with other chaperones (e.g. Hsp70). Cellular location Cytoplasm, Membrane, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:50000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: Jurkat, 2: PC-12, 3: Mouse testis, 4: Rat testis Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 85 kDa Observed band size: 90 kDa IHC-P Tissue: Human testis Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-HSP90 beta Rabbit mAb Clone Number: JRMR-10306-32 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P IP Reactivity: Human, Mouse, Rat Synonyms: HSP 90, HSP90AB1, HSP90B, HSPC2, HSPCB Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms HSP 90, HSP90AB1, HSP90B, HSPC2, HSPCB UniProt ID P08238 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:10000-1:50000 Human, Mouse, Rat IHC-P 1:50 – 1:100 Human, Mouse, Rat IP 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Hsp90 (90 kDa heat shock protein) is a molecular chaperone that aids protein folding and quality control for a large number of ‘client’ proteins. Hsp90 operates as dimer and has intrinsic ATPase activity. The Hsp90 dimer acts in concert with other chaperones (e.g. Hsp70). Cellular location Cytoplasm, Membrane, Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:50000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: Jurkat, 2: PC-12, 3: Mouse testis, 4: Rat testis Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 85 kDa Observed band size: 90 kDa IHC-P Tissue: Human testis Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and highpressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (Blue) Description: The brown color represents the positive signal observed with