anti-hsp70 rabbit mab

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Recombinant Rabbit Monoclonal Anti-HSP70 Rabbit mAb Clone Number: JRMR-10307-5 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P FC Reactivity: Human Synonyms: HSPA1A, HSP72, HSPA1, HSX70 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms HSPA1A, HSP72, HSPA1, HSX70 UniProt ID P0DMV8 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human IHC-P 1:50 – 1:100 Human FC 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Heat shock protein 70 (HSP70) is a ubiquitous and highly conserved protein. As a chaperone molecule, HSP70 can promote the correct folding of proteins; HSP70 participates in cell apoptosis, by preventing the release of cytochrome C from mitochondria, binding with apoptosis-inducing factors to prevent it from entering the nucleus, or inhibiting the activity of JNK kinase to regulate cell apoptosis; HSP70 also It can regulate the cell cycle process, promote cell growth and prevent cell senescence. In addition, HSP70 can be used as an immune adjuvant to participate in synergistic immunity and stimulate antigen-specific CTL responses. Cellular location Cytoskeleton, Cytoplasm, Nucleus, Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: MCF-7, 3: A549Protein loading quantity: 20 μgExposure time: 10 sPredicted band size: 70 kDaObserved band size: 70 kDa IHC-P Tissue: Human breast cancerSection type: Formalin fixed & Paraffin – embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-HSP70 Rabbit mAb Clone Number: JRMR-10307-5 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P FC Reactivity: Human Synonyms: HSPA1A, HSP72, HSPA1, HSX70 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms HSPA1A, HSP72, HSPA1, HSX70 UniProt ID P0DMV8 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human IHC-P 1:50 – 1:100 Human FC 1:50 – 1:100 Human Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Heat shock protein 70 (HSP70) is a ubiquitous and highly conserved protein. As a chaperone molecule, HSP70 can promote the correct folding of proteins; HSP70 participates in cell apoptosis, by preventing the release of cytochrome C from mitochondria, binding with apoptosis-inducing factors to prevent it from entering the nucleus, or inhibiting the activity of JNK kinase to regulate cell apoptosis; HSP70 also It can regulate the cell cycle process, promote cell growth and prevent cell senescence. In addition, HSP70 can be used as an immune adjuvant to participate in synergistic immunity and stimulate antigen-specific CTL responses. Cellular location Cytoskeleton, Cytoplasm, Nucleus, Membrane Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: HeLa, 2: MCF-7, 3: A549Protein loading quantity: 20 μgExposure time: 10 sPredicted band size: 70 kDaObserved band size: 70 kDa IHC-P Tissue: Human breast cancerSection type: Formalin fixed & Paraffin – embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with