anti-hsp70 mouse mab

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Recombinant Anti-HSP70 Mouse mAb Clone Number: 12778 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse Synonyms: HSPA1A, HSP72, HSPA1, HSX70 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms HSPA1A, HSP72, HSPA1, HSX70 UniProt ID P0DMV8 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Hsp70s are a family of conserved ubiquitously expressed heat shock proteins. Proteins with similar structure exist in virtually all living organisms. Intracellularly localized Hsp70s are an important part of the cell’s machinery for protein folding, performing chaperoning functions, and helping to protect cells from the adverse effects of physiological stresses. Additionally, membrane-bound Hsp70s have been identified as a potential target for cancer therapies and their extracellularly localized counterparts have been identified as having both membrane-bound and membrane-free structures. The Hsp70 system interacts with extended peptide segments of proteins as well as partially folded proteins to cause aggregation of proteins in key pathways to downregulate activity. Cellular location Cytoplasm, Cytoskeleton, Nucleus, Secreted Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: NIH/3T3 (42 ℃ heat shock), 2: HeLa, 3: HEK-293 Protein loading quantity: 20 μg Exposure time: 1s Predicted band size: 70 kDa Observed band size: 70 kDa :
Recombinant Anti-HSP70 Mouse mAb Clone Number: 12778 Host: Mouse Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human, Mouse Synonyms: HSPA1A, HSP72, HSPA1, HSX70 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG2a/kappa Conjugate Unconjugated Synonyms HSPA1A, HSP72, HSPA1, HSX70 UniProt ID P0DMV8 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:1000 Human, Mouse Properties Purity Protein G purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Hsp70s are a family of conserved ubiquitously expressed heat shock proteins. Proteins with similar structure exist in virtually all living organisms. Intracellularly localized Hsp70s are an important part of the cell’s machinery for protein folding, performing chaperoning functions, and helping to protect cells from the adverse effects of physiological stresses. Additionally, membrane-bound Hsp70s have been identified as a potential target for cancer therapies and their extracellularly localized counterparts have been identified as having both membrane-bound and membrane-free structures. The Hsp70 system interacts with extended peptide segments of proteins as well as partially folded proteins to cause aggregation of proteins in key pathways to downregulate activity. Cellular location Cytoplasm, Cytoskeleton, Nucleus, Secreted Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Lysate: 1: NIH/3T3 (42 ℃ heat shock), 2: HeLa, 3: HEK-293 Protein loading quantity: 20 μg Exposure time: 1s Predicted band size: 70 kDa Observed band size: 70 kDa