anti-hsp60 rabbit mab

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Recombinant Rabbit Monoclonal Anti-HSP60 Rabbit mAb Clone Number: JRMR-10308-17 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC Reactivity: Human, Mouse, Rat Synonyms: CPN60, HSPD1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CPN60, HSPD1 UniProt ID P10809 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:100 – 1:1000 Human, Mouse ICC/IF 1:1000 – 1:2000 Human FC 1:50 – 1:100 Human, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Heat shock protein 60 (Hsp60), also known as 60 kDa chaperonin (Cpn60), is a highly conserved protein that mainly exists in mitochondria and is very important for key protein folding introduced into mitochondria. When cells are damaged or stressed, HSP60 acts as a danger signal; and HSP60 can be used as an effective stimulator of immune response. HSP60 and its co-chaperone protein HSP10 can assist in the correct folding of nascent polypeptides, prevent misfolding and promote the refolding and correct assembly of unfolded polypeptides produced by the mitochondrial matrix under stress conditions. In addition, changes in the expression level of Hsp60 can be used as a marker for the prognosis or diagnosis of certain cancers and diseases. Cellular location Mitochondria Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb \(HRP Conjugate)Lysate: 1: HeLa, 2: T47D, 3: 293TProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 61 kDaObserved band size: 61 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: NIH-3T3, 2: PC-12Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 61 kDaObserved band size: 61 kDa IHC-P Tissue: Human breast cancerSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with :
Recombinant Rabbit Monoclonal Anti-HSP60 Rabbit mAb Clone Number: JRMR-10308-17 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB IHC-P ICC/IF FC Reactivity: Human, Mouse, Rat Synonyms: CPN60, HSPD1 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms CPN60, HSPD1 UniProt ID P10809 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat IHC-P 1:100 – 1:1000 Human, Mouse ICC/IF 1:1000 – 1:2000 Human FC 1:50 – 1:100 Human, Mouse Properties Purity Protein A purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Heat shock protein 60 (Hsp60), also known as 60 kDa chaperonin (Cpn60), is a highly conserved protein that mainly exists in mitochondria and is very important for key protein folding introduced into mitochondria. When cells are damaged or stressed, HSP60 acts as a danger signal; and HSP60 can be used as an effective stimulator of immune response. HSP60 and its co-chaperone protein HSP10 can assist in the correct folding of nascent polypeptides, prevent misfolding and promote the refolding and correct assembly of unfolded polypeptides produced by the mitochondrial matrix under stress conditions. In addition, changes in the expression level of Hsp60 can be used as a marker for the prognosis or diagnosis of certain cancers and diseases. Cellular location Mitochondria Images WB Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb \(HRP Conjugate)Lysate: 1: HeLa, 2: T47D, 3: 293TProtein loading quantity: 20 μgExposure time: 30 sPredicted band size: 61 kDaObserved band size: 61 kDa Blocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:2000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Lysate: 1: NIH-3T3, 2: PC-12Protein loading quantity: 20 μgExposure time: 60 sPredicted band size: 61 kDaObserved band size: 61 kDa IHC-P Tissue: Human breast cancerSection type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (Blue)Description: The brown color represents the positive signal observed with