anti-hoxb13 rabbit mab

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Recombinant Rabbit Monoclonal Anti-HOXB13 Rabbit mAb Clone Number: JRMR-11054-28 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: Homeobox protein Hox-B13 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Homeobox protein Hox-B13 UniProt ID Q92826 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human Properties Purity Protein A purified Constituents PBS Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background HOXB13 is a member of the HOXB cluster which, along with the HOXA, HOXC, and HOXD clusters, governs embryonic patterning along the cranio-caudal axis. HOXB13 plays a key role in the development of the ventral prostate, where it is expressed highly from the embryonic stage through adulthood. Research studies have shown that both overexpression and RNA interference can inhibit the growth of prostate cancer cells. HOXB13 can function as a tumor suppressor by negatively regulating growth through repression of TCF4 and androgen receptor (AR) signaling. However, HOXB13 has also been shown to be overexpressed in more invasive prostate cancers, breast and ovarian cancers, and hepatocellular carcinomas. A common germline mutation G84E in the HOXB13 protein has recently been found to be associated with significant increased risk of prostate cancer. Currently, HOXB13 is being evaluated as a marker for metastatic lesions of prostate origin. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: LnCap Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 31 kDa Observed band size: 31 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: SW480 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 31 kDa Observed band size: 31 kDa :
Recombinant Rabbit Monoclonal Anti-HOXB13 Rabbit mAb Clone Number: JRMR-11054-28 Host: Rabbit Clonality: Recombinant Monoclonal Applications： WB Reactivity: Human Synonyms: Homeobox protein Hox-B13 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Homeobox protein Hox-B13 UniProt ID Q92826 Immunogen MW (kDa) Specificity Product Usage Information Applications Dilution Recommended Species WB 1:1000 – 1:5000 Human Properties Purity Protein A purified Constituents PBS Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background HOXB13 is a member of the HOXB cluster which, along with the HOXA, HOXC, and HOXD clusters, governs embryonic patterning along the cranio-caudal axis. HOXB13 plays a key role in the development of the ventral prostate, where it is expressed highly from the embryonic stage through adulthood. Research studies have shown that both overexpression and RNA interference can inhibit the growth of prostate cancer cells. HOXB13 can function as a tumor suppressor by negatively regulating growth through repression of TCF4 and androgen receptor (AR) signaling. However, HOXB13 has also been shown to be overexpressed in more invasive prostate cancers, breast and ovarian cancers, and hepatocellular carcinomas. A common germline mutation G84E in the HOXB13 protein has recently been found to be associated with significant increased risk of prostate cancer. Currently, HOXB13 is being evaluated as a marker for metastatic lesions of prostate origin. Cellular location Nucleus Images WB Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: LnCap Protein loading quantity: 20 μg Exposure time: 60 secondsPredicted band size: 31 kDa Observed band size: 31 kDa Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgGH&L (HRP Conjugate) Lysate: 1: SW480 Protein loading quantity: 20 μg Exposure time: 30 secondsPredicted band size: 31 kDa Observed band size: 31 kDa