anti-histone h4 mouse mab

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Anti-Histone H4 Mouse mAb Clone Number: 2F11 Host: Mouse Clonality: Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat, Monkey Synonyms: H4C1, H4/A, H4FA, HIST1H4A, H4C2, H4C3, H4C4, H4C5 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4C1, H4/A, H4FA, HIST1H4A, H4C2, H4C3, H4C4, H4C5 UniProt ID P62805 Immunogen Synthetic peptide corresponding to the N-terminus of human histone H4 protein MW (kDa) 11 Specificity Anti-Histone H4 Mouse mAb detects endogenous levels of total histone H4 protein. It does not cross-react with other histone or non-histone proteins. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat, Monkey IHC-P 1:100 – 1:500 Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are a family of basic proteins that form the core of the nucleosome – the fundamental structural unit of chromatin. In a single nucleosome, core histone proteins H2A, H2B, H3, and H4 form an octamer around which the DNA is tightly wrapped. Histone proteins not only serve to compact chromosomal DNA but also play vital roles in the dynamic and long-term regulation of genes by a wide variety of post-translational modifications (PTMs). These PTMs including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone H4 is primarily acetylated at Lys5, 8, 12, and 16, and methylated at Lys20. Cellular location Nucleus Images WB Lysate: MCF-7, BRL, N2a, COS-7, mouse liver Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 11 kDa Observed band size: 11 kDa Lysate: (-) HeLa; (+) HeLa cells treated with 2 μM trichostatin A for 4 hoursProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 11 kDa Observed band size: 11 kDa IHC-P Tissue: Human neuroblastomaSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with :
Anti-Histone H4 Mouse mAb Clone Number: 2F11 Host: Mouse Clonality: Monoclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat, Monkey Synonyms: H4C1, H4/A, H4FA, HIST1H4A, H4C2, H4C3, H4C4, H4C5 Product Size 100 μl ADD TO CART BUY NOW Quantity Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms H4C1, H4/A, H4FA, HIST1H4A, H4C2, H4C3, H4C4, H4C5 UniProt ID P62805 Immunogen Synthetic peptide corresponding to the N-terminus of human histone H4 protein MW (kDa) 11 Specificity Anti-Histone H4 Mouse mAb detects endogenous levels of total histone H4 protein. It does not cross-react with other histone or non-histone proteins. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat, Monkey IHC-P 1:100 – 1:500 Human Properties Purity Protein G and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are a family of basic proteins that form the core of the nucleosome – the fundamental structural unit of chromatin. In a single nucleosome, core histone proteins H2A, H2B, H3, and H4 form an octamer around which the DNA is tightly wrapped. Histone proteins not only serve to compact chromosomal DNA but also play vital roles in the dynamic and long-term regulation of genes by a wide variety of post-translational modifications (PTMs). These PTMs including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone H4 is primarily acetylated at Lys5, 8, 12, and 16, and methylated at Lys20. Cellular location Nucleus Images WB Lysate: MCF-7, BRL, N2a, COS-7, mouse liver Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 11 kDa Observed band size: 11 kDa Lysate: (-) HeLa; (+) HeLa cells treated with 2 μM trichostatin A for 4 hoursProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Mouse IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 11 kDa Observed band size: 11 kDa IHC-P Tissue: Human neuroblastomaSection type: Formalin fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1:500Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter stain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with