anti-histone h3 rabbit pab

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Anti-Histone H3 Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat, Monkey Synonyms: Histone H3.1, Histone H3/a, H3C1, H3FA, HIST1H3A, Histone H3/b, Histone H3/c, Histone H3/d Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Histone H3.1, Histone H3/a, H3C1, H3FA, HIST1H3A, Histone H3/b, Histone H3/c, Histone H3/d UniProt ID P68431 Immunogen Synthetic peptide corresponding to the C-terminus of human histone H3 protein MW (kDa) 15 Specificity Anti-Histone H3 Rabbit pAb detects endogenous levels of total histone H3 protein. It does not cross-react with other histone or non-histone proteins. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat, Monkey IHC-P 1:100 – 1:500 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are a family of basic proteins that form the core of the nucleosome – the fundamental structural unit of chromatin. In a single nucleosome, core histone proteins H2A, H2B, H3, and H4 form an octamer around which the DNA is tightly wrapped. Histone proteins not only serve to compact chromosomal DNA but also play vital roles in the dynamic and long-term regulation of genes by a wide variety of post-translational modifications (PTMs). These PTMs including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Among the histone members, histone H3 is the most extensively modified and its amino-terminal tail structure has been shown to be especially critical for eukaryotic cells. Cellular location Nucleus Images WB Lysate: HeLa, C2C12, COS-7, rH3Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 15 kDa Observed band size: 17 kDa Lysate: Mouse liver, mouse heartProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 15 kDa Observed band size: 17 kDa Lysate: (-) Hela; (+) Hela cells treated with 30 mM sodium butyrate for 4 hoursProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 15 kDa Observed band size: 17 kDa IHC-P Tissue: Human neuroblastoma Section type: Formalin-fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with :
Anti-Histone H3 Rabbit pAb Clone Number: / Host: Rabbit Clonality: Polyclonal Applications： WB IHC-P Reactivity: Human, Mouse, Rat, Monkey Synonyms: Histone H3.1, Histone H3/a, H3C1, H3FA, HIST1H3A, Histone H3/b, Histone H3/c, Histone H3/d Product Size 100 μl Shipping: Ambient temperature Order online or send purchase order to [email protected] FAQ Technical Support Protocols General Information Product Usage Information Properties Target Information Images Recommended Products References BUY NOW General Information Isotype IgG Conjugate Unconjugated Synonyms Histone H3.1, Histone H3/a, H3C1, H3FA, HIST1H3A, Histone H3/b, Histone H3/c, Histone H3/d UniProt ID P68431 Immunogen Synthetic peptide corresponding to the C-terminus of human histone H3 protein MW (kDa) 15 Specificity Anti-Histone H3 Rabbit pAb detects endogenous levels of total histone H3 protein. It does not cross-react with other histone or non-histone proteins. Product Usage Information Applications Dilution Recommended Species WB 1:500 – 1:2000 Human, Mouse, Rat, Monkey IHC-P 1:100 – 1:500 Human Properties Purity Protein A and immunogen affinity purified Constituents PBS, Glycerol, BSA Storage Store at -20°C. Avoid freeze/thaw cycles. Stability Stable for 12 months from date of receipt/reconstitution. Target Information Background Histones are a family of basic proteins that form the core of the nucleosome – the fundamental structural unit of chromatin. In a single nucleosome, core histone proteins H2A, H2B, H3, and H4 form an octamer around which the DNA is tightly wrapped. Histone proteins not only serve to compact chromosomal DNA but also play vital roles in the dynamic and long-term regulation of genes by a wide variety of post-translational modifications (PTMs). These PTMs including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Among the histone members, histone H3 is the most extensively modified and its amino-terminal tail structure has been shown to be especially critical for eukaryotic cells. Cellular location Nucleus Images WB Lysate: HeLa, C2C12, COS-7, rH3Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 15 kDa Observed band size: 17 kDa Lysate: Mouse liver, mouse heartProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 15 kDa Observed band size: 17 kDa Lysate: (-) Hela; (+) Hela cells treated with 30 mM sodium butyrate for 4 hoursProtein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 secondsPredicted band size: 15 kDa Observed band size: 17 kDa IHC-P Tissue: Human neuroblastoma Section type: Formalin-fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use) Counter stain: Hematoxylin (blue) Description: The brown color represents the positive signal observed with