Product Name :
anti-histone h3 rabbit mab
Isotype :
IgG
Conjugate :
Unconjugated
Synonyms:
Histone H3.1, Histone H3/a, H3C1, H3FA, HIST1H3A, Histone H3/b, Histone H3/c, Histone H3/d
UniProt ID :
P68431
Immunogen:
Synthetic peptide corresponding to the N-terminus of human histone H3 protein
MW (kDa) :
15
Specificity:
Anti-Histone H3 Rabbit mAb detects endogenous levels of total histone H3 protein. It does not cross-react with other histone or non-histone proteins.
Purity :
Protein A purified
Purity :
PBS, Glycerol, BSA
Storage :
Store at -20°C. Avoid freeze/thaw cycles.
Stability:
Stable for 12 months from date of receipt/reconstitution.
Background :
Histones are a family of basic proteins that form the core of the nucleosome – the fundamental structural unit of chromatin. In a single nucleosome, core histone proteins H2A, H2B, H3, and H4 form an octamer around which the DNA is tightly wrapped. Histone proteins not only serve to compact chromosomal DNA but also play vital roles in the dynamic and long-term regulation of genes by a wide variety of post-translational modifications (PTMs). These PTMs including acetylation, methylation, phosphorylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Among the histone members, histone H3 is the most extensively modified and its amino-terminal tail structure has been shown to be especially critical for eukaryotic cells. Cellular location Nucleus
Images :
WB Lysate: 1: HeLa, 2: NIH-3T3, 3: BRL, 4: Rat kidney, 5: Mouse kidney, 6: Rat brain, 7: Mouse brainProtein loading amount: 20 μgBlocking buffer: 5% NFDM/TBSTPrimary Ab dilution: 1:1000Primary Ab incubation condition: 2 hours at room temperatureSecondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate)Exposure time: 30 secondsPredicted band size: 15 kDaObserved band size: 15 kDa IHC-P Tissue: Human prostate hyperplasiaSection type: fixed & paraffin-embedded sectionRetrieval method: High temperature and high pressureRetrieval buffer: Tris/EDTA buffer, pH 9.0Primary Ab dilution: 1: 500Primary Ab incubation condition: 1 hour at room temperatureSecondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to Use)Counter strain: Hematoxylin (blue)Description: The brown color represents the positive signal observed with
Vapor Pressure :
Recombinant Rabbit Monoclonal Anti-Histone H3 Rabbit mAb Clone Number: PA
Recombinant Rabbit Monoclonal Anti-Histone H3 Rabbit mAb Clone Number: PA
Related websites: https://www.medchemexpress.com/antibodies.html
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