Was into Rd, Rg3, compound K, and other compounds [26]. Moreover, the crude enzyme extract from Leuconostoc citreum can convert ginsenoside Rb1 within the following sequence: Rb1RdF2compound K [27]. Consistently, -glucosidase isolated from D. anomala YAE-1 fully converted ginsenoside Rb1 to ginsenoside Rd right after 48 h of incubation [14].Foods 2022, 11, 529 Foods 2022, 11,of 13 88ofFigure 7. Conversion of ginsenoside Rb1 by crude enzyme from Lactobacillus buchneri URN103L: Figure 7. Conversion of ginsenoside Rb1 by crude enzyme from Lactobacillus buchneri URN103L: (A) (A) 0 days; (B) three days; (C) 7 days; (D) 10 days; (E) 14 days. 0 days; (B) three days; (C) 7 days; (D) ten days; (E) 14 days.three.7. Characteristics of the Fermentation of 20 Ginseng Root Solution by L. buchneri URN103L 3.7. Qualities in the Fermentation of 20 Ginseng Root is shown in Table 1. URN103L The viable cell count of fermented ginseng roots Answer by L.Oxyntomodulin supplier buchneri L. buchneriThe viable cell count at 3 . The initial viable cell count just Table 1. L. buchneri URN103L was inoculated of fermented ginseng roots is shown inafter inoculation was URN103L was inoculated at three . colony-forming unit (CFU) mL-1 . Just after 7 days of ferapproximately 6.04 0.077 log The initial viable cell count just just after inoculation was around six.FIPI Metabolic Enzyme/Protease,Autophagy 04 0.PMID:23557924 077 count increased to 8.27 0.044 log .CFU mL-1 and fermentamentation, the viable cell log colony-forming unit (CFU) mL-1 Following 7 days of decreased tion, the 0.083cell count mL-1 more than 14 days.0.044 six typesmLLAB in fermented ginseng by 7.39 viable log CFU increased to eight.27 The log CFU of -1 and decreased by 7.39 0.083 logapproximately 9 11 log CFU mL-1 sorts of LAB in fermented ginseng decreased grew by CFU mL-1 over 14 days. The six on the first day, and immediately after 5 days, they grew by to 6.0 7.5 log 9 11 log [2]. Strain L. buchneri URN103L converted they decreased to around CFU mL-1CFU mL-1 around the very first day, and following 5 days, key ginsenoside Rb1 to minor ginsenosides Rd L. buchneri URN103L converted in Figure eight. TLC Rb1 to six.0 7.five log CFU mL-1 [2]. Strain and Rg3 (epimer R/S), as shownmajor ginsenosideanalysis showed that the extent of conversion increased shown in Consequently, the growth showed minor ginsenosides Rd and Rg3 (epimer R/S), aswith time. Figure eight. TLC evaluation time was enhanced to a of conversion improved with time. Thus, the growth time enzyme that the extent 14-day period, such that there might be elevated production in the was in-glucosidase. Figure 9A shows the outcomes of fermented production of root. When L. creased to a 14-day period, such that there may well be increasedPanax ginsengthe enzyme buchneri URN103L in shows the outcomes was incubated, ginsenosides Rb1 and L. have been glucosidase. Figure 9A 20 ginseng root of fermented Panax ginseng root. When Rdbuch-Foods 2022, 11,olized into Rg3 just after 3 days (Figure 9B). Subsequently, just after 7 days, ginsenoside R gradually appeared (Figure 9C). As shown in Figure 9D, the resultant minor ginse Rg5, Rd, and Rg3 started to become detected, and their content material elevated more than time. Am 17 sorts of LAB exhibiting optimistic -glucosidase activity from fermentation pro 9 of 13 Korean plant foods, only 1 (i.e., URN103L) showed high hydrolytic activity on g side Rb1. The LAB strain was identified as L. buchneri URN103L, which showed metabolized into Rg3 soon after three NRRLB 30929. The optimum hydrolysis activity in the mology with L. buchneri days (Figure 9B). Subsequently, immediately after 7 days, ginsenoside Rg3.
