ntributions NM, GJD, HSO, and JGB developed and planned the investigation. MH ready fungal cultures. CB and SS ready activitybased probes utilised within this study. NM collected secretome samples and performed activitybased protein profil ing experiments. NM collected and analysed proteomic information. DN performed bioinformatic analysis. NM and MS prepared P. pastoris strains, produced and purified IL-1 Storage & Stability recombinant enzymes, and performed activity assays. NM wrote the manuscript with input from all the authors. All authors study and authorized the final manuscript. Funding The authors thank the Organic Sciences and Engineering Study Council of Canada (PostDoctoral Fellowship to NGSM), the Royal Society (Ken Murray Research Professorship to GJD), the Biotechnology and Biological Sciences Research Council (BBSRC) (grant BB/R001162/1 to GJD), the French National Study Agency (ANR13BIME0002 to JGB), the Netherlands Organization for Scientific Research (NWO Top grant 2018714.018.002 to HSO), and also the European Analysis Council (ERC2011AdG290836 “Chembiosphing” to HSO, ERC2020SyG951231 “Carbocentre” to GJD and HSO). Proteomics information were collected at the York Centre of Excellence in Mass Spectrometry, which was designed thanks to a significant capital investment by means of Science City York, sup ported by Yorkshire Forward with funds from the Northern Way Initiative, and HSP90 Storage & Stability subsequent help from EPSRC (EP/K039660/1; EP/M028127/1). Availability of data and components Pichia pastoris strains and samples of recombinant proteins may be offered from Gideon Davies ([email protected]). Samples of ABPCel, ABPXyl, and ABPGlc may be accessible from Herman Overkleeft (h.s.overkleeft@lic. leidenuniv.nl). Basidiomycete fungi are available from the fungal culture collection in the International Centre of Microbial Sources (CIRMCF) at the French National Institute for Agricultural analysis (INRA; Marseille, France). Genome sequences for each and every on the fungi applied in this study are obtainable from Mycocosm (mycocosm.jgi.doe.gov/mycocosm/home) (DOE Joint Genome Institute, Walnut Creek, California). Other datasets applied and/or ana lysed in the course of the existing study are available from the corresponding author on affordable request.Author information 1 York Structural Biology Laboratory, Department of Chemistry, The University of York, Heslington YO10 5DD, York, UK. two Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA Leiden, The Netherlands. three UMR1163 Bio diversitet Biotechnologie Fongiques, Facultdes Sciences de Luminy, INRAE, Aix Marseille Univ, 13288 Marseille, France. four Polytech Marseille, Aix Marseille Univ, 13288 Marseille, France. Received: eight October 2021 Accepted: 6 JanuaryDeclarationsEthics approval and consent to participate Not applicable. Consent for publication Not applicable. Competing interests The authors declare no competing interests.References 1. Scheller HV, Ulvskov P. Hemicelluloses. Annu Rev Plant Biol. 2010;two(61):2639. two. Luis AS, Briggs J, Zhang X, Farnell B, Ndeh D, Labourel A, et al. Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018;3(two):210. 3. Celiska E, Nicaud JM, Bialas W. Hydrolytic secretome engineering in Yarrowia lipolytica for consolidated bioprocessing on polysaccharide resources: evaluation on starch, cellulose, xylan, and inulin. Appl Microbiol Biotechnol. 2021;105(three):9759. 4. Schlembach I, Hosseinpour Tehrani H, Blank LM, B hs J, Wierckx N, Regestein L, et al. Consolidate
