Product Name: IL-4 Antibody
Species Reactivity: Human
Tested Applications: ELISA, Neut, WB
Applications: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-4 (1.50 ng/mL), a concentration of 0.06 -0.1 μg/mL of this antibody is required. ELISA:To detect hIL-4 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 – 0.4 ng/well of recombinant hIL-4. Sandwich:To detect hIL-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 – 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-4 (XP-5188Bt) as a detection antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-4.Western Blot:To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-4 (human Interleukin-4).
Host Species: Rabbit
Purification: Anti-hIL-4 specific antibody was purified by affinity chromatography employing immobilized hIL-4 matrix.
Physical State: Lyophilized
CAS NO.: 34031-32-8
Product: Ellipticine
Buffer:
Concentration:
Storage Conditions: IL-4 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: BSF1, IL-4, BCGF1, BSF-1, BCGF-1Interleukin-4, B-cell stimulatory factor 1
Accession NO.: P05112
Protein Ino: 124337
Official Symbol: IL4
Geneid: 3565
Background:
PubMed ID:http://aac.asm.org/content/26/4/498.abstract
